| 黑米花色苷提取物对大鼠抗过敏作用 |
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| 引用本文： | 李良昌,延光海,秦向征,沈光海. 黑米花色苷提取物对大鼠抗过敏作用[J]. 中国公共卫生, 2011, 27(5) |
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| 作者姓名： | 李良昌 延光海 秦向征 沈光海 |
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| 作者单位： | 1. 延边大学医学部解剖学教研部,吉林延吉,133000
2. 延边大学医学部药学院 |
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| 基金项目： | 延边大学人才引进博士科研启动基金(2009第44号) |
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| 摘 要： | 目的观察黑米花色苷提取物(anthocyanin-rich extract from black rice,AEBR)的抗过敏作用并探讨其可能的作用机制。方法通过IgE诱导大鼠被动皮肤过敏反应(PCA)实验,用比色法测定黑米花色苷提取物在体内对肥大细胞影响;体外实验观察黑米花色苷提取物对IgE诱导组胺释放、细胞内钙摄入及其他炎性介子的影响。结果动物实验显示,AEBR高、中剂量组(300、150 mg/kg)明显抑制PCA实验,抑制率分别49.71%、30.40%。细胞实验显示,AEBR高、中剂量组(100、50μg/L)明显抑制组胺的释放(抑制率分别为75.56%、44.76%)、细胞内钙摄入(抑制率分别为64.75%、44.56%)以及肿瘤坏死因子-α(TNF-α)[黑米花色苷提取物(25、50、100μg/L)给药10 min使TNF-α蛋白表达分别下降7.8%、80%与85%]、白细胞介素6(IL-6)[黑米花色苷提取物(25、50、100μg/L)给药10min使IL-6蛋白表达分别下降5.6%,77%与84.1%]的释放(P<0.05)。结论黑米花色苷提取物抗过敏作用与抑制肥大细胞脱颗粒及炎性介子的...
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| 关 键 词： | 肥大细胞 黑米花色苷提取物 免疫球蛋白E 组胺 钙 |
Anti-allergic effects of anthocyanin-rich extract from black rice |
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| LI Liang-chang,YAN Guang-hai,QIN Xiang-zheng,et al.. Anti-allergic effects of anthocyanin-rich extract from black rice[J]. Chinese Journal of Public Health, 2011, 27(5) |
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| Authors: | LI Liang-chang YAN Guang-hai QIN Xiang-zheng et al. |
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| Affiliation: | LI Liang-chang,YAN Guang-hai,QIN Xiang-zheng,et al.Department of Anatomy,School of Basic Medical Sciences,College of Pharmacy,Yanbian University(Yanji 133000 China) |
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| Abstract: | Objective To investigate the anti-allergic effects of anthocyanin-rich extract from black rice(AEBR) and to explore its possible mechanism.Methods The experiment of passive cutaneous anaphylaxis(PCA)was used to determine the effect of AEBR on degranulation of mast cells in vivo.For in vitro study,various doses of AEBR were added to sensitized rat peritoneal mast cells(RPMC).The IgE-induced release of degranulation was measured by enzymatic assay,histamine by enzyme immunoasassay(EIA),and cytokines by enzyme... |
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| Keywords: | mast cell AEBR IgE histmaine calcium |
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