薯蓣皂苷元对人低分化胃腺癌细胞株生长的抑制作用

目的：观察薯蓣皂苷元（diosgenin, Dio）对人低分化胃腺癌细胞株（MGC-803）的细胞分裂和集落形成的影响，初步探讨其杀伤肿瘤细胞的机制。 方法：采用四唑蓝（MTT）比色法、平板集落形成实验以及［³H］-TdR掺入实验。结果：MTT法检测Dio作用24、48、72 h的半数抑制浓度(IC₅₀)为56.290、27.837、17.723 mg?L^-1；平板集落形成实验显示Dio半数集落形成抑制浓度为5.486 mg?L^-1；Dio在较低浓度（3.750和7.500 mg?L^-1）下，可直接抑制MGC-803细胞DNA的合成。结论：Dio能够明显抑制MGC-803的细胞分裂和集落形成。

Inhibitory effect of diosgenin on human poorly differentiated gastric adenocarcinoma cell line

ObjectiveTo study the effect of diosgenin on the cell diversion and colony forming of human poorly differentiated gastric adenocarcinoma cell line MGC 803,and investigate the mechanism of cell death induced by diosgenin. Methods MTT assay,colony forming experiment and 3H] TdR incorporation into DNA were used. ResultsAfter incubated with diosgenin for 24, 48, and 72 hours, the IC 50 were 56 290, 27 837, and 17 723 mg·L -1 ,respectively, detected by MTT. The concentration which inhibited half colony forming was 5 486 mg·L -1 . It directly inhibited the synthesis of DNA of MGC 803 in lower concentrations (3 750 and 7 500 mg·L -1 ). ConclusionDiosgenin has an obvious inhibitory effect on the cell diversion and colony forming of MGC 803.