| 光动力学疗法影响人工菌斑生物膜形成的扫描电镜观察 |
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| 引用本文： | 许小辉,林居红,刘明方. 光动力学疗法影响人工菌斑生物膜形成的扫描电镜观察[J]. 第三军医大学学报, 2010, 32(21) |
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| 作者姓名： | 许小辉 林居红 刘明方 |
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| 作者单位： | 重庆医科大学附属口腔医院牙体牙髓科,重庆,400015;重庆医科大学实验教学中心,重庆,400016 |
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| 摘 要： | 目的 研究甲苯胺蓝介导的光动力学疗法对体外人工菌斑生物膜形成的影响,探讨光动力学疗法控制菌斑和防治龋病的可能机制. 方法 体外环境下,4种口腔常见致龋菌在牙釉质表面形成多细菌混合性菌斑生物膜,用光动力学疗法处理实验组菌斑生物膜,光敏剂浓度为0.1 mg/ml、光强为94.5 J/cm2;分别在生物膜形成的第1、3、7天用扫描电镜观察光动力学疗法处理对生物膜形成的影响.结果 对照组第1天可见到以链球菌为主的细菌黏附于获得性膜上;第3天各种细菌数量明显增多,形成谷穗状结构;至第7天时,生物膜形成复杂的三维立体结构,细菌表面及细菌之间可见大量基质和不定形胶状物.在第1、3、7天,光动力学疗法处理后牙釉质表面黏附的细菌显著减少,菌斑生物膜形成受阻.结论 光动力学疗法对菌斑生物膜的形成有抑制作用.
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| 关 键 词： | 光动力学疗法 龋病 菌斑生物膜 扫描电镜 |
Effect of photodynamic therapy on biological membrane formation of artificial dental plaques |
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| Xu Xiaohui,Lin Juhong,Liu Mingfang. Effect of photodynamic therapy on biological membrane formation of artificial dental plaques[J]. Acta Academiae Medicinae Militaris Tertiae, 2010, 32(21) |
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| Authors: | Xu Xiaohui Lin Juhong Liu Mingfang |
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| Affiliation: | Xu Xiaohui 1,Lin Juhong 1,Liu Mingfang2(1Department of Endodontics,Affiliated Stomatology Hospital,Chongqing Medical University,Chongqing,400015,2Experimental Teaching Center,400016,China) |
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| Abstract: | Objective To study the effect of toluidine blue-mediated photodynamic therapy on biological membrane formation of dental plaques and its mechanism for the control of dental plaques and prevention of dental caries.Methods Biological membrane of dental plaques caused by 4 cariogenic oral bacteria was treated with photodynamic therapy.Concentration of the photosensitizer was 0.1 mg/mL and the light intensity was 94.5 J/cm2.Effect of photodynamic therapy on biological membrane formation of dental plaques was ob... |
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| Keywords: | photodynamic therapy dental caries biological membrane of dental plaque scan electron microscope |
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