Glutamine attenuates TPN-associated liver injury in infant rabbits |
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Authors: | Jiang Wu Li Hong Wei Cai Qingya Tang Chenren Shi |
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Institution: | (1) Clinical Nutrition Center, Department of Pediatric Surgery, Xin Hua Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai, 200092, China |
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Abstract: | The aim of this study was to assess the effects of parenteral alanyl-glutamine dipeptide (Ala-Gln) on TPN-associated liver
injury. Forty-three New Zealand rabbits (6–8 days old) were divided into three groups: 12 in the control group (maternal fed);
18 in the TPN group (TPN for 10 days); 13 in the Gln-PN group (TPN+Ala-Gln 400 mg kg−1 day−1 for 10 days). At the end of the experiment, liver function and histology were evaluated; MDA content of liver tissues and
hepatocyte apoptosis by TUNEL assay were also determined. The serum concentration of direct bilirubin and bile acid in the
Gln-PN group was significantly lower than TPN group (P < 0.05), but showed no difference compared with the control group. AST level of the Gln-PN group was lower than the other
two groups. The light microscopy (LM) features in the TPN group included cholestasis or diffuse steatosis, while in the Gln-PN
group, inflammatory infiltration and mild hydropic degenerative changes were mainly found without obvious cholestasis or proliferation
of bile ducts. The electron microscopy appearances corresponded with LM findings. The liver MDA content in the Gln-PN group
was clearly lower than the TPN group (P < 0.05), and was lower without statistical significance compared with control group. TUNEL assays showed the ratio of apoptotic
hepatocytes in the TPN group was the highest among all the groups (44.59 ± 6.68 vs. 0.92 ± 0.85 in the control group, P < 0.01; 44.59 ± 6.68 vs. 4.14 ± 2.76 in the Gln-PN group, P < 0.01). There were significantly fewer apoptotic hepatocytes in the Gln-PN group. From this study, we found that glutamine
dipeptide supplementation could attenuate TPN-associated liver injury in infant rabbits, and could also decrease liver MDA
production and hepatocyte apoptosis during total parenteral nutrition.
This study was supported in part by the National Key Program Grant (No.2004BA709B09). |
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Keywords: | Total parenteral nutrition Hepatic/liver dysfunction Glutamine Apoptosis |
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