| 高迁移率族蛋白A在雄性小鼠生殖细胞株中的表达 |
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| 引用本文： | 马征兵,杨瑛,王珺,李磊.高迁移率族蛋白A在雄性小鼠生殖细胞株中的表达[J].中华男科学杂志,2009,15(6):534-537. |
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| 作者姓名： | 马征兵 杨瑛 王珺 李磊 |
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| 作者单位： | 第四军医大学唐都医院妇产科,陕西,西安,710038 |
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| 摘 要： | 目的:检测高迁移率族蛋白A(HMGA)在雄性小鼠生殖细胞株TM4、GC-1spg、GC-2spd(ts)中的表达,为深入探讨HMGA基因在小鼠精子发生过程中的作用机制奠定理论基础。方法:应用RT-PCR、Western印迹检测雄性小鼠生殖细胞株TM4、GC-1spg、GC-2spd(ts)中HMGA1、HMGA2基因的表达。结果:HMGA1mRNA、HMGA2mRNA在雄性小鼠生殖细胞株TM4、GC-1spg、GC-2spd(ts)中均有不同程度表达,Western印迹结果表明HMGA1与HMGA2蛋白在雄性小鼠生殖细胞株TM4、GC-1spg、GC-2spd(ts)中呈阳性表达,与RT-PCR的结果一致。结论:HMGA的表达可能参与了细胞株TM4、GC-1spg、GC-2spd(ts)的分裂、增殖,在雄性小鼠精子发生过程中可能发挥着重要作用。
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| 关 键 词： | 高迁移率族蛋白A 精子发生 小鼠 |
Expression of High Mobility Group Protein A in Male Mouse Testicular Cell Lines |
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| MA Zheng-bing,YANG Ying,WANG Jun,LI Lei.Expression of High Mobility Group Protein A in Male Mouse Testicular Cell Lines[J].National Journal of Andrology,2009,15(6):534-537. |
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| Authors: | MA Zheng-bing YANG Ying WANG Jun LI Lei |
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| Institution: | MA Zheng-bing,YANG Ying,WANG Jun,LI Lei Department of Obstetrics , Gynecology,Tangdu Hospital,The Fourth Military Medical University,Xi'an,Shaanxi 710038,China |
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| Abstract: | Objective:To detect the expression of high mobility group protein A (HMGA) in male mouse testicular cell lines TM4, GC-1spg and GC-2spd(ts), and to pave the theoretical ground for further investigation of the action mechanism of the HMGA gene in male mouse spermatogenesis. Methods: We detected the expressions of HMGA1 and HMGA2 by RT-PCR and Western blot in the male mouse testicular cell lines TM4, GC-1spg and GC-2spd(ts). Results: HMGA1 and HMGA2 were expressed in the male mouse testicular cell lines TM4, ... |
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| Keywords: | high mobility group protein A spermatogenesis mouse |
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