| RP-HPLC测定腺苷蛋氨酸注射用溶剂中赖氨酸的含量及有关物质 |
| |
| 引用本文： | 赵鹏,王东凯,庄润,薛梅妍,王丽君,黎玲. RP-HPLC测定腺苷蛋氨酸注射用溶剂中赖氨酸的含量及有关物质[J]. 中国药学杂志, 2006, 41(13): 1023-1025 |
| |
| 作者姓名： | 赵鹏 王东凯 庄润 薛梅妍 王丽君 黎玲 |
| |
| 作者单位： | 1. 沈阳药科大学药学院,沈阳,110016
2. 沈阳沃森药物研究所,沈阳,110016 |
| |
| 摘 要： | 目的建立测定腺苷蛋氨酸注射用溶剂中赖氨酸含量及有关物质的方法。方法采用DiamonsilTM(钻石)C18ODS柱(4.6mm×250 mm,5μm);流动相为甲醇-水-三乙胺(250∶750∶0.1),流速为0.8 mL·min-1,紫外检测波长为210 nm,柱温为30℃。结果在本色谱条件下L-赖氨酸与辅料及溶剂峰分离度符合要求,在300～700 mg·L-1内线性良好(r=0.999 7)。结论测定方法简便、准确、灵敏度高、方法可靠,可作为质量控制方法。
|
| 关 键 词： | 赖氨酸 反相高效液相色谱法 含量测定 有关物质 |
| 文章编号： | 1001-2494（2006）13-1023-04 |
| 收稿时间： | 2005-06-29 |
| 修稿时间： | 2005-06-29 |
Determination of Lysine and Related Compounds by RP-HPLC |
| |
| ZHAO Peng,WANG Dong-kai,ZHUANG Run,XUE Mei-yan,WANG Li-jun,LI Ling. Determination of Lysine and Related Compounds by RP-HPLC[J]. Chinese Pharmaceutical Journal, 2006, 41(13): 1023-1025 |
| |
| Authors: | ZHAO Peng WANG Dong-kai ZHUANG Run XUE Mei-yan WANG Li-jun LI Ling |
| |
| Affiliation: | 1.School of Pharmacy，Shenyang Pharmaceutical University，Shenyang 110016，China； 2.Shenyang Watson Pharmaceutical Institute，Shengyang 110016，China |
| |
| Abstract: | OBJECTIVE To establish a RP-HPLC method for the determination of Lysine and related compounds.METHODS The separation was performed with a DiamonsilTM C18 ODS columon(4.6 mm×250 mm,5 μm) at 30 ℃,and the mobile phase consisted of methanol-water-triethylamine(250∶750∶0.1) at the flow rate of 0.8 mL·min-1.UV detection wavelength was at 210 nm.RESULTS A good linearity was obtained over the range of 300～700 mg·L-1(r=0.999 7).CONCLUSION This method is simple,accurate,sensitive and applicable for the quality control of this preparation. |
| |
| Keywords: | Lysine RP-HPLC determination related compounds |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国药学杂志》浏览原始摘要信息 |
|
点击此处可从《中国药学杂志》下载免费的PDF全文 |
|
