| 褪黑素对谷氨酸所致大鼠神经细胞Ca~(2+)内流的影响 |
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| 引用本文： | 杨红英,贾孟良,李经才,董淳. 褪黑素对谷氨酸所致大鼠神经细胞Ca~(2+)内流的影响[J]. 中国药房, 2005, 16(3): 174-176 |
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| 作者姓名： | 杨红英 贾孟良 李经才 董淳 |
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| 作者单位： | 1. 深圳市人民医院药学部,深圳市,518020
2. 沈阳药科大学,沈阳市,110015 |
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| 摘 要： | 目的 :研究褪黑素对谷氨酸引起的神经细胞Ca2 +内流的抑制作用。方法 :用Ca2 +敏感荧光指示剂Fura -2/AM负载大鼠脑细胞 ,测定神经细胞内游离Ca2 +浓度。结果 :谷氨酸500μmol/L可促进Ca2 +内流 ,显著增加细胞内游离Ca2 +浓度 (P<0. 01) ;应用褪黑素10 -5mol/L后可显著抑制Ca2 +内流 ,降低细胞内游离Ca2 +浓度 (P<0. 01)。结论 :褪黑素可通过抑制谷氨酸引起的Ca2 +内流保护神经细胞。
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| 关 键 词： | 褪黑素 谷氨酸 神经细胞 Ca2+ |
| 文章编号： | 1001-0408(2005)03-0174-03 |
| 修稿时间： | 2004-07-09 |
Effects of Melatonin on the Influx of Ca2+ Induced by L- glutamate in Cultured Rat Neurons |
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| YANG Hongying,JIA Mengliang,DONG Chun. Effects of Melatonin on the Influx of Ca2+ Induced by L- glutamate in Cultured Rat Neurons[J]. China Pharmacy, 2005, 16(3): 174-176 |
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| Authors: | YANG Hongying JIA Mengliang DONG Chun |
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| Abstract: | OBJECTIVE:To study the inhibitory effect of melatonin(MT)on the increased influx of Ca 2+ induced by L-glutamate in cultured rat neurons.METHODS:Fura-2/AM,a Ca 2+ sensitive fluorescent indicator was used to load the brain cells isolated from new-born rats,and the intracellular dissociative Ca 2+ concentration(〔Ca 2+ 〕i)was measured with fluoresˉcence-spectrophotometer.RESULTS:The L-glutamate of concentration500μmol/L could markedly promote the influx of Ca 2+ and intracellular free Ca 2+ concentration(P<0.01),while10 -5 mol/L MT added to L-glutamate could significantly inˉhibit the influx and decrease the concentration.CONCLUSION:MT could protect the cultured rat neurons by inhibiting the influx of Ca 2+ induced by L-glutamate. |
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| Keywords: | Ca2 |
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