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Multi-target stool DNA test in the surveillance of inflammatory bowel disease: a cross-sectional cohort study |
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| Authors: | Pasquale Klepp John B Kisiel Milada Cvancarova Småstuen Arne Røseth Solveig N Andersen Morten H Vatn |
| Institution: | 1. Department of Internal Medicine, Unger-Vetlesen Institute, Lovisenberg Diaconal Hospital, Oslo, Norway;2. Institute of Clinical Medicine, University of Oslo, Oslo, Norway;3. pasklepp@gmail.com;5. Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA;6. Institute of Clinical Medicine, University of Oslo, Oslo, Norway;7. Department of Gastroenterology, Oslo University Hospital, Oslo, Norway;8. Department of Internal Medicine, Lovisenberg Diaconal Hospital, Oslo, Norway;9. Department of Pathology, Akershus University Hospital, L?renskog, Norway |
| Abstract: | Background and aim: Colonoscopic surveillance is recommended in patients with longstanding inflammatory bowel disease (IBD) as they are at increased risk of colorectal cancer (CRC). Non-invasive surveillance may improve compliance and access. Multi-target stool DNA (MT-sDNA) has been validated for screening of sporadic CRC but has not been assessed in IBD. Our aim was to assess the performance of a MT-sDNA test in a real-life surveillance setting of patients with longstanding IBD.Material and methods: A total of 192 IBD patients enrolled from two prospective cohorts submitted an EDTA buffered stool sample and underwent chromo- or white light colonoscopy. Stools were assayed for methylated BMP3 &; NDRG4, mutant KRAS and β-actin by a laboratory blinded to clinical data.Results: The multitarget-sDNA panel was positive in 2/2 CRC and 5/15 low-grade dysplasia (LGD)?Conclusion: The MT-sDNA panel detected CRC in IBD. Sensitivity for sub-centimeter colorectal neoplasms in IBD patients appears similar to that observed in the general population. The test may be a valuable tool for detection of malignancy during structured surveillance of long-term IBD in a first line hospital setting. |
| Keywords: | Inflammatory bowel disease colorectal cancer screening diagnostic tests stool DNA test |