| Bcl-2在小鼠肾脏发生发育中的表达 |
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| 引用本文： | 宋小峰,陈雪,任昊,翟效月. Bcl-2在小鼠肾脏发生发育中的表达[J]. 解剖学报, 2012, 43(5): 658-661. DOI: 10.3969/j.issn.0529-1356.2012.05.014 |
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| 作者姓名： | 宋小峰 陈雪 任昊 翟效月 |
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| 作者单位： | 1.中国医科大学组织学与胚胎学教研室，沈阳 110001; 2.辽宁医学院组织学与胚胎学教研室，辽宁 锦州 121001 |
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| 基金项目： | 国家自然科学基金资助项目，辽宁省自然科学基金资助项目 |
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| 摘 要： | 目的 探讨小鼠肾脏发育过程中抗凋亡蛋白Bcl-2的表达. 方法 选取胚龄16(E16d)、18d和出生后1d(P1d)、3、5、7d、14和21d的昆明小白鼠共54只,应用免疫组织化学技术并结合体视学分析方法,观察小鼠肾脏不同发育时期树脂切片上Bcl-2的表达. 结果 在小鼠肾脏发育过程中,Bcl-2的阳性表达主要出现在近端小管,从E18 d到P1 d,Bcl-2阳性表达肾小管面数密度的增长速度最快,在P7d之后,Bcl-2阳性表达肾小管面数密度的增长速度的变化不大. 结论 在肾的早期发育过程中,Bcl-2主要影响了近端小管的存活或死亡.
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| 关 键 词： | Bcl-2 近端小管 发育 树脂切片 免疫组织化学 免疫荧光 小鼠 |
| 收稿时间： | 2011-10-23 |
Expression of Bcl-2 during the development of mouse kidney |
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| SONG Xiao-feng , CHEN Xue , REN Hao , ZHAI Xiao-yue. Expression of Bcl-2 during the development of mouse kidney[J]. Acta Anatomica Sinica, 2012, 43(5): 658-661. DOI: 10.3969/j.issn.0529-1356.2012.05.014 |
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| Authors: | SONG Xiao-feng CHEN Xue REN Hao ZHAI Xiao-yue |
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| Affiliation: | 1.Department of Histology and Embryology,China Medical University, Shenyang 110001,China;(2.Department of Histology and Embryology, Liaoning Medical College, Liaoning Jinzhou 121001, China |
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| Abstract: | Objective To explore the expression of Bcl-2 during mouse kidney development. Methods Fifty-five Kunming mice from embryonic day 16(E16d), 18 and postnatal day 1(P1d), 3, 5, 7, 14, 21 were chosen to be used to investigate the expression of Bcl-2 on epoxy sections with immunohistochemistry technique and stereological methods. Results During mouse kidney development, the positive staining of Bcl-2 was mainly observed in proximal tubules. The increase with numerical area density(N A) of Bcl-2 was the fastest from E18d to P1d, while not much changed after P7 d. Conclusion During the pristine kidney development, the expression of Bcl-2 influences survival or death of proximal tubule. |
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| Keywords: | Bcl-2 Proximaltubule Development Epoxysection Immunohistochemistry Immunofluorescence Mouse |
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