小剂量辣椒素对大鼠胃缺血再灌注胃黏膜的保护作用

目的研究小剂量辣椒素（capsaicine，Cap）对大鼠胃缺血再灌注（gastric ischemia-reperfusion,GI-R）后胃黏膜损伤的保护作用。方法sD大鼠18只随机分为假手术（Sham）组、GI．R组（又分为Cap组和Cap对照组）3组，每组6只。Cap组和Cap对照组于GI-R模型建立24h后、Sham组于假手术24h后，取大鼠胃组织。按Guth标准计数胃黏膜损伤指数；HE染色行组织病理学观察。结果GI—R后胃黏膜弥漫性充血水肿及点片状出血糜烂，Cap组较Cap对照组表现轻。Sham组、Cap组和Cap对照组胃损伤指数分别为：3．20±1．81、31．12±12．13和60．25±23．76，3组比较差异有统计学意义（P〈0．01，P〈0．05）。HE染色组织病理学检查，Cap组较Cap对照组病理损伤明显减轻。结论小剂量Cap能降低GI—R大鼠的胃黏膜损伤指数，对胃有明显的保护作用。

The protective effect of minidose capsaicine on gastric mucosa injury of gastric ischemia-reperfusion in rats

Objective To investigate the protective effect of minidose capsaicine（Gap） on gastric mucosa injury after gastric ischemia-reperfusion in rats. Methods Sprague-Dawley rats were randomly divided into sham- operated （Sham） group and gastric ischemia-reperfusion （GI-R） group, and the latter was randomly divided into two groups： Cap group and Cap control group （n =6,each group）. Rats were sacrificed 24 h after GI-R model established or sham-operated, and gastric tissue was obtained. Gastric mucosal injury index was counted and standard by Guth under stereomicroscope. Histopathological changes in the gastric mucosa were investigated by HE staining under lightmicroscope. Results After GI-R, diffuse mucosal edema and bleeding point sheet erosion was found un- der stereomicroscope. Compare to Cap control group, Cap group exhibited mild injury. Guth scores of Sham group, Cap group and Cap control group were 3.20 ±1.81,31.12 ± 12.13 and 60.25 ±23.76, respectively. Score difference among the three groups was statistically significant （ P 〈 0.01, P 〈 0. 05 ）. The pathological damage of Cap group was milder than Cap control group by HE staining. Conclusion Application of minidose Cap can reduce the gastric mucosal injury index in GI-R.