IgM抗体激活补体可致ABO血型误判的探讨

为了正确确定ABO血型和保证输血的安全，收集了两名献血员的血液样品，应用红细胞凝集试验和抗人球蛋白试验研究对ABO血型鉴定中抗体激活补体的影响。结果表明：两个ABO血型样品中正向型和反向型不相符，抗C3血样均为阳性，抗IgG血样均为阴性。结论：正向型和反向型不相符是由于抗IgM抗体激活补体所致，两例血型经试验最终定为O型。本方法对正确鉴定ABO血型有意义。

ABO Typing Error Caused by Anti-IgM Activated Complements

In order to determine the appraisal of ABO blood group accurately and ensure the safety of blood transfusion,an investigation was made on the influence of complement activated by antibody in appraising ABO blood group by using hemoagglutination test and direct antiglobulin test. Two samples of ABO blood group were collected from two donors. The results showed that the obverse and reverse patterns did not accord each other in two samples of ABO blood group ,and their blood samples were both positive for anti-C_3 and were both negative for anti-IgG. It is concluded that the incosistency of the obverse and reverse patterns in two samples of ABO blood group were proved to be caused by the complements activated by the antibody against IgM. Blood group O was finally determined for the two samples,and the influences of IgM antibody and complement on ABO blood group were excluded when the test proceeded. This method will be useful to determine ABO blood group accurately.