氧环境对血管内皮生长因子、色素上皮衍生因子的影响及其与血管发育的关系

目的：研究视网膜发育过程中血管内皮生长因子(VEGF)和色素上皮衍生因子(PEDF)所起的调节作用及其机制。方法：C57BL/6J 小鼠从出生后7～12 d 饲养于75%氧环境,分别用抗 VEGF、抗 PEDF 和抗 CD31抗体作免疫组织化学标记视网膜切片。结果：持续处于高氧环境时,VEGF 表达处于低水平而 PEDF 快速上升,血管生长减缓。高氧处理5 d 后回到普通环境,VEGF 的表达迅速上升而 PEDF 却逐渐减少,出现了一过性的血管快速增长现象。结论：氧含量变化可调节 VEGF 和 PEDF 表达,参与视网膜血管发育。

Effect of oxygen on vascular endothelial growth factor, pigment epithelium derived factor and its relation with retinal vascularization

Objective:To determine the role of vascular endothelial growth factor(VEGF)and pigment epithelium derived factor(PEDF)in vascularization of the developing retina.Methods:Retinas were harvested at different postnatal days from C57BL/6J mice that have been maintained at ambient oxygen or exposed to 75% oxygen from P7 to P12.Immunostaining of VEGF,PEDF and CD31 was performed.Results:When the mice were exposed to 75% oxygen,the expression of VEGF was inhibited and expression of PEDF greatly enhanced;vascularization was slowed down.When exposed to the normal air condition 5 days later,the expression of VEGF increased sharply and peaked at P17.Conclusion:Oxygen can regulate the expression of VEGF and PEDF,and is involved in vascular development in retina.