罗格列酮对单侧输尿管梗阻大鼠肾脏的抗氧化保护作用

目的:观察新型胰岛素增敏剂罗格列酮对单侧输尿管梗阻(UUO)的肾间质损伤的抗氧化保护作用.方法:将雄性SD大鼠随机分成三大组:假手术对照组(Sham组,n=6)、UUO模型组(结扎左侧输尿管制作UUO模型,n=18)、UUO罗格列酮干预组(UUO RSG组,n=18).除Sham组外,其余2组根据不同的观察时间细分为3、7、14 d亚组(,n=6).分别测定大鼠左肾皮质匀浆中脂质过氧化物标志物MDA以及抗氧化酶CAT、SOD含量.结果:与Sham组相比,UUO模型组大鼠梗阻侧肾皮质在3、7、14 d时MDA含量分别升高了8.9(P=0.000)、3.5(P=0.000)和0.7倍,而CAT含量分别下降了66.0%、72.6%和83.8%(P＝0.000),SOD分别下降了42.0%、50.2%和67.7%(P<0.005).与UUO模型组相比,UUO RSG组大鼠梗阻侧皮质MDA含量在3、7 d 时分别减少了80.0%和65.3%(P=0.000),14 d时差异不显著;在3、7、14 d时CAT分别增加了96.2%、92.1%和102.9%(P<0.005),SOD分别增加了33.3%、35.1%和55.4%(P<0.005).结论:RSG能减少单侧输尿管梗阻侧肾皮质脂质过氧化物的产生,同时增加抗氧化酶的含量,从而改善单侧输尿管梗阻大鼠氧化应激.

Antioxidative effects of rosiglitazone on rat kidney with unilateral ureteral obstruction

Objective:To investigate the antioxidative effect of rosiglitazone(RSG),a new insulin sensitizer,on kidney of rats with unilateral ureteral obstruction(UUO).Methods: Male adult Sprague-Dawley rats were randomly divided into 3 groups: Sham-operated group(n=6),UUO model group(UUO model was established by ligating the left ureter,n=18),and UUO+RSG group(UUO model rats were treated with RSG,n=18).The latter 2 groups were divided into subgroups according to the times(3 d,7 d and 14 d) after intervention(n=6).Malondialdehyde(MDA),catalase(CAT) and superoxide dismutase(SOD) contents in the homogenized lysate of obstructive renal cortex were determined.Results: Compared with those in Sham-operated group,MDA contents in UUO group increased by 8.9(P=0.000),3.5(P=0.000) and 0.7 times on d 3,d 7 and d 14,respectively,while the contents of CAT and SOD decreased dramatically,with CAT contents reduced by 66.0%,(72.6%) and 83.8%,respectively(P=0.000) and SOD contents reduced by 42.0%,50.2% and 67.7%,respectively(P<(0.005).) Compared with those in UUO model group,MDA contents in UUO+RSG group decreased by 80.0% and 65.3% on d 3 and d 7(P=0.000),respectively;no significant difference was found between the 2 contents on d 14;CAT contents were increased by 96.2%,92.1% and 102.9%,respectively(P<0.005) and SOD content were increased by 33.3%,35.1% and 55.4%((P<)0.005),respectively.Conclusion: Rosiglitazone can suppress oxidative stress in rats with UUO by reducing lipid peroxidation production and improving antioxidative enzyme contents.