| 丹酚酸A对缺氧/复氧损伤条件下脑微血管内皮细胞ICAM-1表达的影响 |
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| 引用本文： | 姜民,郭利平,周王怡,李晶,王婕.丹酚酸A对缺氧/复氧损伤条件下脑微血管内皮细胞ICAM-1表达的影响[J].天津中医药,2008,25(5):406-407. |
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| 作者姓名： | 姜民 郭利平 周王怡 李晶 王婕 |
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| 作者单位： | 天津中医药大学研究生,天津,300193 |
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| 摘 要： | 目的]通过对观察丹酚酸A对缺氧/复氧(H/R)损伤条件下脑微血管内皮细胞细胞间黏附因子-1(CAM-1)表达的影响,探讨丹酚酸A对缺血再灌注急性期脑保护作用机制.方法]培养BMEC,丹酚酸A预处理20 h后制作(H/R)/模型,逆转录-聚合酶链式反应(RT-PCR)法检测BMEC细胞间黏附因子-1 ICAM-1 mRNA的表达.以尼莫地平作为阳性对照药.结果]模型组与空白对照组相比ICAM-1 mRNA表达明显升高(P<0.01).丹酚酸A组ICAM-1 mRNA表达低于模型组(P<0.05).结论]丹酚酸A可降低H/R损伤条件下BMEC ICAM-1 mRNA表达的影响,提示阻抑粒细胞黏附作用是其脑保护作用机制之一.
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| 关 键 词： | 丹酚酸A 缺氧/复氧(H/R) 细胞间黏附因子-1(ICAM-1) 逆转录-聚合酶链式反应(RT-PCR) |
| 收稿时间： | 5/6/2008 12:00:00 AM |
Effect of salvianolic acid A on ICAM-1 expression after Hypoxia/ Reoxygenation injury in brain microvascular endothelial cells |
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| JIANG Min,GUO Li-ping,ZHONG Wang-yi.Effect of salvianolic acid A on ICAM-1 expression after Hypoxia/ Reoxygenation injury in brain microvascular endothelial cells[J].Tianjin Journal of Traditional Chin Medicine,2008,25(5):406-407. |
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| Authors: | JIANG Min GUO Li-ping ZHONG Wang-yi |
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| Institution: | JIANG Min,GUO Li-ping,ZHONG Wang-yi,et al (Tianjin University of TCM,Tianjin 300193,China) |
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| Abstract: | Objective] To investigate the action of Sal A on the expression of cellular adhesion molecules after hypoxia/reoxygenation (H/R) injury in brain microvascular endothelial cells (BMEC). Methods] After pretreating with Sal A for 20h, the H/R was successfully established H/R on BMEC. Then the expression of ICAM-mRNA was evaluated by RT-PCR method. Nimodipine served as positive control.Results] Compared with control, the expression of ICAM-mRNA of H/R model enhanced significantly (P<0.01). Sal A could downre... |
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| Keywords: | Salvianolic Acid A hypoxia/reoxygenation ICAM-1 RT-PCR |
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