| 红车轴草提取物对小鼠T淋巴细胞体外活化的抑制作用 |
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| 引用本文： | 杨志,曾耀英,黄秀艳,李林,姚满林,宋兵,滕菲.红车轴草提取物对小鼠T淋巴细胞体外活化的抑制作用[J].现代免疫学,2008,28(5). |
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| 作者姓名： | 杨志 曾耀英 黄秀艳 李林 姚满林 宋兵 滕菲 |
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| 作者单位： | 暨南大学组织移植与免疫实验中心,广州,510632 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，广东省科研项目，广东省广州市科技局科技攻关项目 |
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| 摘 要： | 探讨红车轴草提取物(Trifolium pratense Leguminosae extract,TLE)对小鼠T淋巴细胞的体外活化的影响。无菌条件下制备小鼠淋巴细胞悬液;双色荧光抗体染色结合流式细胞术分别分析TLE对小鼠T淋巴细胞在刀豆蛋白A(ConA)或佛波醇酯(PDB)刺激下的体外分化抗原CD69、CD25、CD71表达的影响。终质量浓度为20、30、40 mg/L的TLE对小鼠T淋巴细胞在ConA或PDB刺激下的体外分化抗原CD69、CD25、CD71表达具有明显的抑制作用(P<0.01)。TLE对ConA或PDB刺激的不同时期的小鼠T淋巴细胞的体外活化具有明显的抑制作用。
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| 关 键 词： | 红车轴草提取物 T淋巴细胞 CD69 CD25 CD71 |
Inhibitory effect of Trifolium pratense leguminosae extract on the in vitro activation of mouse T lymphocytes |
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| YANG Zhi,ZENG Yao-ying,HUANG Xiu-yan,LI Lin,YAO Man-lin,SONG Bing,TENG Fei.Inhibitory effect of Trifolium pratense leguminosae extract on the in vitro activation of mouse T lymphocytes[J].Current Immunology,2008,28(5). |
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| Authors: | YANG Zhi ZENG Yao-ying HUANG Xiu-yan LI Lin YAO Man-lin SONG Bing TENG Fei |
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| Abstract: | To investigate the influence of Trifolium pretense legumnosae extract(TLE) on the in vitro activation of mouse T lymphocytes,the mouse lymphocyte suspension was prepared under sterile condition and the effect of TLE on the expressions of the in vitro differential antigens CD69,CD25 and CD71 on T lymphocytes stimulated by ConA or PDB were analyzed by means of double-fluorescent staining plus flow cytometry.It was found that TLE at the mass concentration of 20,30 and 40 μg/ml showed marked inhibition on the expressions of CD69,CD25 and CD71 in T lymphocytes(P<0.01).This result indicates that TLE exhibits suppressive role on the in vitro activation of T lymphocytes. |
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| Keywords: | CD69 CD25 CD71 |
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