依达拉奉对大鼠脑缺血再灌注损伤的保护作用

目的:观察依达拉奉对大鼠脑缺血再灌注损伤的影响,并探讨其可能的作用机制。方法:制备SD大鼠MCAO模型,梗死2h后再灌注,并分为假手术组、生理盐水(NS)组、依达拉奉低剂量组及依达拉奉高剂量组,再灌注24h后进行神经功能缺损评分及检测各组脑组织超氧化物歧化酶(SOD)、丙二醛(MDA)浓度。结果:①依达拉奉低剂量与高剂量组的神经功能缺损评分明显低于NS组(P<0.01),且依达拉奉高济量组的神经功能缺损评分明显低于低剂量组(P<0.01);②与假手术组相比,NS组SOD活性降低、MDA含量增加,有显著性差异(P<0.01);与NS组相比,依达拉奉低剂量与高剂量组SOD活性增高、MDA含量降低(P<0.01),且低剂量组与高剂量组相比有显著性差异(P<0.01)。结论:依达拉奉通过降低自由基水平,对脑缺血再灌注损伤有保护作用。

Protection of Edaravone against Cerebral Ischemia Reperfusion Injury in Rats

Objective:To investigate effects of Edaravone on cerebral ischemia-reperfusion injury.Methods:Cerebral ischemia-reperfusion model was generated by occluding the right middle cerebral artery(MCA)for 2 h and followed by reperfusion in rats.The rats were randomly divided into sham operated group,normal saline(NS)group,low dose of Edaravone-treating group and high dose of Edaravone-treating group.After 24 h of reperfusion,the neurologic deficit scores of rats were documented and the content of super oxide dismutase(SOD),and malondialdehyde(MDA)were measured.Results:①The neurologic deficit scores of low dose of Edaravone-treating group and high dose of Edaravone-treating group were significantly less than that in NS group rats(P<0.01).②Compared with sham operation group,the content of SOD in NS group was significantly decreased and the content of MDA was obviously increased(P<0.01).The content of SOD in the low dose of Edaravone-treating group and the high dose of Edaravone-treating group was significantly elevated and MDA was decreased than in the NS group(P<0.01).And there were difference between the low dose and high dose of Edaravone group.Conclusion:Edaravone may reduce free radicals to lower cerebral ischemia-reperfusion injury.