| 大环内酯类药物对肺炎链球菌的防耐药突变浓度的研究 |
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| 引用本文： | 邢茂,刘同华,王琴.大环内酯类药物对肺炎链球菌的防耐药突变浓度的研究[J].第三军医大学学报,2009,31(13). |
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| 作者姓名： | 邢茂 刘同华 王琴 |
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| 作者单位： | 1. 第三军医大学新桥医院药剂科,重庆,400037
2. 重庆市中医研究院药物研究部,重庆,400013 |
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| 摘 要： | 目的 测定大环内酯类药物对肺炎链球菌的防耐药突变浓度(mutant prevention concentration,MPC),扩增突变选择窗(mutant selection window,MSW)内筛选的耐药突变体的耐药基因,以了解肺炎链球菌对大环内酯类药物耐药的发生机制.方法 肉汤法富集1010CFU/ml ATCCA9619,采用平板二倍稀释法测定罗红霉素和阿奇霉素对ATCC49619的最低抑菌浓度(minimal inhibitory concentration,MIC)、MIC99、初测MPC(Provisional MPC,MPCpr)以及MPC.PCR法扩增不同药物筛选出的耐药突变株的耐药基因ermB和mefA并测序.结果 罗红霉素和阿奇霉素对ATCC49619的MPC分别为0.80μg/ml和0.51 μg/ml,细菌耐药选择指数(MPC/MIC99)为5.0和3.9.筛选出的耐药突变株中扩增出ermB耐药基因.结论 通过凋整药物剂量,可以限制对大环内酯类耐药的肺炎链球菌突变体的富集.肺炎链球菌对大环内酯类药物耐药机制可能与携带ermB基因有关.
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| 关 键 词： | 防耐药变异浓度 罗红霉素 阿奇霉素 肺炎链球菌 |
Study of mutant prevention concentrations of macrolide-resistant Streptococcus pneumoniae |
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| XING Mao,LIU Tong-hua,WANG Qin.Study of mutant prevention concentrations of macrolide-resistant Streptococcus pneumoniae[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(13). |
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| Authors: | XING Mao LIU Tong-hua WANG Qin |
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| Institution: | XING Mao1,LIU Tong-hua1,WANG Qin2 (1Department of Pharmacy,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,2Department of Drug Research,Chongqing Institute of Traditional Chinese Medicine,Chongqing 400013,China) |
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| Abstract: | Objective To detect the mutant prevention concentration (MPC) of macrolide resistant Streptococcus pneumoniae, to amplify the resistance gene of Streptococcus pneumoniae mutants selected in the mutant selection window (MSW), and to investigate the mechanism in the resistance of Streptococcus pneumoniae to macrolides. Methods The Streptococcus pneumoniae strain ATCC49619 was enriched in broth, and the bacterial concentrations were adjusted to 1010 colony forming units per milliliter. The minimal inhibitory c... |
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| Keywords: | mutant prevention concentration roxithromycin azithromycin Streptococcus pneumoniae |
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