益气健脾抗癌中药对胃癌裸鼠肝肾脏蛋白激酶C影响的实验研究

目的探讨益气健脾抗癌中药对胃癌裸鼠肝脏和肾脏蛋白激酶C活性的影响。方法在40只裸小鼠中随机选取30只腋下接种人胃癌SGC一7901细胞株，将成瘤裸鼠随机分为模型组、补脾对照组和补脾抗癌组，每组10只，剩余10只未种瘤裸鼠设为空白组。空白组和模型组予0．9％NaCl溶液灌胃，补脾对照组予益气健脾中药灌胃，补脾抗癌组予益气健脾合抗癌中药灌胃。各组给予相应的干预措施14天，观察动物的肝肾组织大体情况并称重，计算脏器系数，用WesternBlot法检测肝脏和肾脏PKC活性。结果与空白组比较，模型组瘤鼠肝系数、。肾系数降低，差异有统计学意义（P〈0．05）；与模型组比较，补脾对照组和补脾抗癌组肝系数、肾系数升高，差异有统计学意义（P〈0．05）。同时模型组肝脏和肾脏PKC活性较空白组明显升高，补脾对照组和补脾抗癌组裸鼠肝脏和肾脏中PKC活性较模型组均下降（P〈0．01），其中补脾抗癌组较补脾对照组下降更为显著（P〈0．01）。结论胃癌瘤鼠存在脾虚症状，益气健脾和益气健脾抗癌中药对胃癌裸鼠体质量、肝重、肾重、肝系数、肾系数，以及肝脏和肾脏中PKC活性均具有调节作用，且益气健脾抗癌中药对PKC的调节作用优于益气健脾中药。

Chinese medicine with replenishing qi and invigorating spleen effects on protein kinase C activity in liver and kidney of nude mice with gastric cancer

Objective To discuss the effect of Chinese medicine on protein kinase C （PKC） activity in liver and kidney of nude mice with stomach cancer. Methods Thirty nude mice out of 40 were selected randomly and armpit inoculated with human gastric cancer cells SGC-7901. The nude mice were randomly divided into model group, tonifying spleen control group and tonifying spleen anticancer group, with 10 mice in each group. The rest of 10 mice were divided into blank group. The mice in blank group and model group were treated with normal saline, tonifying spleen control group was treated with Chinese medicine with replenishing qi effect, tonifying spleen anticancer group was treated with Chinese medicine with combined replenishing qi and anticancer effects, with 0.8 ml each and for 14 days by garage. The condition of the liver and kidney tissue were observed and the weights were measured. The organ coefficients were counted and the PKC activities in liver and kidney were tested by Western Blot method. Results Compared with blank group, the liver coefficient and kidney coefficient in model group were decreased, with significant difference between the two groups （ P 〈 0.05 ） ; compared with model group, the liver coefficient and kidney coefficient in tonifying spleen control group and tonifying spleen anticancer group were increased significantly （ P 〈 0.05 ）. The PKC activities in liver and kidney of the model group were significantly higher than that of the blank group. After treatment, the PKC activities in liver and kidney of the tonifying spleen group and tonifying spleen anticancer group were lower than that of the model group, and the PKC activity in tonifying spleen anticancer group was lower than that of the tonifying spleen control group significantly （ P 〈 0.01 ）. Conclusion Spleen deficiency symptom is very common in mice with gastric cancer. Chinese medicine has regulatory effect on the body weight, fiver weight, kidney weight, liver coefficient, kidney coefficient and PKC activities in nude mice. Moreover, the Chinese medicine with replenishing spleen qi and anticancer effects has better regulatory effect on PKC activities than that with replenishing spleen qi effect.