| 苍术薄层色谱指纹图谱研究 |
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| 引用本文： | 黄琦,陈炎明,侴桂新,陈静.苍术薄层色谱指纹图谱研究[J].上海中医药杂志,2013(6):107-109. |
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| 作者姓名： | 黄琦 陈炎明 侴桂新 陈静 |
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| 作者单位： | [1]上海健康职业技术学院,上海200237 [2]上海中药标准化研究中心,上海201203 [3]上海市食品药品检验所,上海201203 |
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| 基金项目： | 国家中医药管理局中医药科技重大项目（02代08）; 上海市重点学科建设项目（Y0301）; 上海市科委科研计划项目（03DZ19546） |
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| 摘 要： | 目的建立中药苍术的薄层色谱指纹图谱。方法采用硅胶GF254预制板以及硅胶HSGF254预制板,控制室内温度25℃、相对湿度53%,展开剂组成为甲苯-乙酸乙酯（7∶1）,紫外光（254 nm）下观察荧光猝灭斑点。用薄层色谱视频摄像系统拍摄图像,并自动生成色谱轮廓扫描图谱,同时获得积分数据。并对收集的10批药材样品在同一薄层板上平行分析比较。结果不同地区的10批苍术药材的薄层色谱指纹图谱有共同的特征荧光色谱图像。结论苍术的薄层色谱指纹图谱可作为其指纹特征。
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| 关 键 词： | 苍术 薄层色谱 指纹图谱 |
Study on thin-layer chromatographic fingerprint of Rhizoma Atractylodis |
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| HUANG Qi,CHEN Yan-Ming,CHOU Gui-Xin,CHEN Jing.Study on thin-layer chromatographic fingerprint of Rhizoma Atractylodis[J].Shanghai Journal of Traditional Chinese Medicine,2013(6):107-109. |
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| Authors: | HUANG Qi CHEN Yan-Ming CHOU Gui-Xin CHEN Jing |
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| Institution: | 1.Shanghai Health Vocational ＆Technical College;2.Shanghai Research and Development Centre for Standardization of Chinese Medicines;3.Shanghai Institute for Food and Drug Control |
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| Abstract: | Objective To establish the thin-layer chromatographic（TLC） fingerprint of Rhizoma Atractylodis.Methods Use silica gel HSGF254 and GF254,the indoor temperature is 25 ℃,relative humidity 53%,developing agent composition for toluene-ethyl acetate（7 ∶1）,and the fluorescent quenching spots were observed under UV（254 nm）.By TLC picture pick-up system,the fingerprint was scanned and the data was acquired.Ten batches of samples in the same thin-layer plate were parallel analyzed and compared.Results The TLC fingerprint of 10 batches of Rhizoma Atractylodis collected from different regions had common characteristics of fluorescence image.Conclusion The TLC fingerprint can be used as a unique fingerprint characteristics of Rhizoma Atractylodis. |
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| Keywords: | Rhizoma Atractylodis thin-layer chromatography（TLC） fingerprint |
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