The cloning and sequencing of thealcB gene,coding for alcohol dehydrogenase II,inAspergillus nidulans |
| |
Authors: | Gary D. Hunter I. Gwyn Jones Heather M. Sealy-Lewis |
| |
Affiliation: | (1) Department of Applied Biology, University of Hull, HU6 7RX Hull, UK |
| |
Abstract: | Alcohol dehydrogenase II (ADH II, structural genealcB) was purified from a strain H1035,biA1; alcE1; alc500 alcD1, which produces 100-times more ADH II activity than thealcAalcR deletion strain (alc500). Antibodies were raised against this ADH, and were used to screen a cDNA library in gt11. We have isolated the gene for an ADH which is over-expressed in H1035, and which we believe to be thealcB gene; cDNA and genomic clones were sequenced. The sequence contains three introns and encodes a protein of 367 amino acids. This protein shows a clear level of identity to a range of alcohol dehydrogenases, but is no more closely related to the ADH I and ADH III previously described inA. nidulans than to the ADHs ofS. pombe andS. cerevisiae. The significance of consensus sequences found in the 5 region of the gene is discussed in relation to the regulation of the gene. |
| |
Keywords: | Alcohol dehydrogenase II Aspergillus nidulans |
本文献已被 SpringerLink 等数据库收录! |
|