Chk1表达与食管鳞癌浸润转移的关系

目的 探讨Chk1表达与食管鳞癌浸润转移的关系。方法 采用Western Blot、RT-PCR技术分别检测62例食管鳞癌组织、31例癌旁不典型增生组织及62例正常食管黏膜组织中Chk1蛋白及mRNA的表达,探讨Chk1表达与食管鳞状细胞癌浸润转移之间的关系。结果 正常食管黏膜组织、癌旁不典型增生组织以及食管鳞癌组织中,Chk1蛋白、mRNA的表达水平依次升高,三者间两两比较差异均有统计学意义（P〈0.05）;深层浸润组食管鳞癌组织中Chk1蛋白、mRNA的表达水平均显著高于浅层浸润组,两组比较差异有统计学意义（P〈0.05）;有淋巴结转移组的食管鳞癌组织中Chk1蛋白、mRNA的表达水平显著高于无淋巴结转移组,两组比较差异有统计学意义（P〈0.05）。结论 Chk1高表达与食管鳞癌浸润转移有关。

Relationship between expression of Chk1 and invasion and metastasis in esophageal squamous cell carcinoma

Objective To investigate The relationship between expression of Chk1 and iInvasion and metastasis in esophageal squamous cell carcinoma（ESCC）. Methods Using Western blot,in situ hybridization RT- PCR methods to detect the expression of Chk1 gene in 62 cases of esophageal squamous cell carcinoma,31 cases of adjacent dysplasia and62 cases of normal esophageal mucosa. Results The expression level of Chk1 protein and mRNA gradually increased from normal esophagus mucous tissue,adjacent atypical hyperplasia tissueto ESCC tissue,and there was significant difference among three groups（P〈0. 05）. The expression of Chk1 mRNA and protein in the deep layer invaded groups were considerably higher than those in infiltrating shallow layer group,and there was significant difference among the groups（P〈0. 05）. The expressions of Chk1 mRNA and protein in lymphatic metastasis groups were significantly higher than those without lymphatic metastasis group,and there was significant difference among the groups（P〈0. 05）. Conclusion The expression of Chk1 protein and mRNA were closely associated with the invasion and metastasis of ESCC.