| DIFFERENCES IN EXPRESSION AND REGULATION BETWEEN TRANSFORMED CELLS OF THE HUMAN GASTRIC CARCINOMA ONCOGENE Ha-ras AND THE UNTRANSFORMED PARENT CELLS |
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| 作者姓名： | 韩复生 刘淑萍 宋建国 袁艳华 张维 施华 邓国仁 刘培楠 |
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| 作者单位： | Department of Biochemistry and Molecular Biology，Beijing Institute for Cancer Research，Department of Biochemistry，Inner Mongolia Medical College，Huhhot，Department of Biochemistry and Molecular Biology，Beijing Institute for Cancer Research，Department of Biochemistry and Molecular Biology，Beijing Institute for Cancer Research，Department of Biochemistry and Molecular Biology，Beijing Institute for Cancer Research，Department of Biochemistry and Molecular Biology，Beijing Institute for Cancer Research，Department of Biochemistry and Molecular Biology，Beijing Institute for Cancer Research，Department of Biochemistry and Molecular Biology，Beijing Institute for Cancer Research |
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| 摘 要： | An Ha-ras oncogene was isolated from a cell line of gastric carcinoma called BGE-823 in order to elucidate genetic control and the influence of DNA sequences. The oncogene was cloned and identified as a single nucleotide substitution of thymine for guanine in the 12th codon through the sequencing of its first axon. We compared the differences of expression and regulation between the transformed Ha-ras cells and untransformed parent cells. Data indicated that the expression of Ha-ras in the transformed cells was five-fold higher than in the untransformed cells and that the Ha-ras gene in the former was hypersensitive toward DNase I. In addition, a nuclear protein of 35 kilodaltons bound strongly to the 2.5 Kb fragment located upstream of the 6.6 Kb Ha-ras gene and contained a CC rich region. These results suggest that there might be another mechanism of activation for the ras gene besides point mutation.
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