Tityusγ toxin,a high affinity effector of the Na+ channel in muscle,with a selectivity for channels in the surface membrane |
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Authors: | Jacques Barhanin Michèle Ildefonse Oger Rougier Suely Vilela Sampaio José R. Giglio Michel Lazdunski |
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Affiliation: | (1) Faculté des Sciences, Centre de Biochimie du CNRS, Parc Valrose, F-06034 Nice Cedex, France;(2) C.N.R.S. L.A. 244, Laboratoire de Physiologie des Eléments Excitables, Université Claude Bernard, 43 Bd. du 11 novembre 1918, F-69621 Villeurbanne Cedex, France;(3) Faculdade de Medicine de Ribeirao Preto, Universidade de Sao Paulo, BR-14100 Ribeirao Preto, Sao Paulo, Brazil |
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Abstract: | Toxin from the venom ofTityus serrulatus scorpion produces a partial block of the surface Na+ channel in frog muscle. This block occurs with no change in the voltage-dependence or in the kinetics of the remaining surface Na+ current. The partial blockade of Na+ channel activity occurs with no change in tubular Na+ currents nor in twitch tension. The maximum effect of the toxin is attained at concentrations as low as 3×10–10 M. Hyperpolarization to potentials more negative than the resting potential (E=–90 mV) reduces or abolishes the effect of the toxin.Radioiodinated toxin binds to frog muscle membranes with a very high affinity corresponding to a dissociation constant of about 1×10–11 M. Data obtained with both rabbit and frog muscle indicate that toxin is specific for Na+ channels in surface membranes. Toxin does not seem to bind to Na+ channels in T-tubule membranes. The biochemical data are in good agreement with electrophysiological studies and data on contraction. There is oneTityus toxin binding site per tetrodotoxin binding site in surface membranes. Competition experiments have confirmed thatTityus toxin binds to a new toxin receptor site on the Na+ channel structure. This site is the same that the toxin II fromCentruroides suffusus binding site, but this toxin has 100 times less affinity for the Na+ channel thanTityus toxin. |
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Keywords: | Scorpion neurotoxin Ion transport Muscle excitation-contraction coupling Receptor binding |
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