Mesenchymal stem cell‐conditioned medium accelerates wound healing with fewer scars |
| |
| Authors: | Meirong Li Fuxin Luan Yali Zhao Haojie Hao Jiejie Liu Liang Dong Xiaobing Fu Weidong Han |
| |
| Affiliation: | 1. Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Science, Chinese PLA General Hospital, Beijing, P. R. China;2. Trauma Treatment Center, Central Laboratory, Chinese PLA General Hospital Hainan Branch, Sanya, P. R. China |
| |
| Abstract: | Mesenchymal stem cells (MSCs) derived from umbilical cord s (UC‐MSCs) have been shown to enhance cutaneous wound healing by means of the paracrine activity. Fibroblasts are the primary cells involved in wound repair. The paracrine effects of UC‐MSCs on dermal fibroblasts have not been fully explored in vitro or in vivo. Dermal fibroblasts were treated with conditioned media from UC‐MSCs (UC‐MSC‐CM). In this model, UC‐MSC‐CM increased the proliferation and migration of dermal fibroblasts. Moreover, adult dermal fibroblasts transitioned into a phenotype with a low myofibroblast formation capacity, a decreased ratio of transforming growth factor‐β1,3 (TGF‐β1/3) and an increased ratio of matrix metalloproteinase/tissue inhibitor of metalloproteinases (MMP/TIMP). Additionally, UC‐MSC‐CM‐treated wounds showed accelerated healing with fewer scars compared with control groups. These observations suggest that UC‐MSC‐CM may be a feasible strategy to promote cutaneous repair and a potential means to realise scarless healing. |
| |
| Keywords: | Fibroblast Mesenchymal stem cell‐conditioned medium Scarless TGF‐β |
|
|
