| PMX诱导神经管畸形及其对神经上皮细胞增殖和凋亡的影响 |
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| 引用本文： | 关涛,王秀伟,官臻,王建华,王国亮,朱智强,谢秋,牛勃. PMX诱导神经管畸形及其对神经上皮细胞增殖和凋亡的影响[J]. 神经解剖学杂志, 2011, 27(4): 447-452 |
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| 作者姓名： | 关涛 王秀伟 官臻 王建华 王国亮 朱智强 谢秋 牛勃 |
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| 作者单位： | 1. 山西医科大学生物化学与分子生物学教研室,太原,030001
2. 首都儿科研究所,北京,100020 |
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| 摘 要： | 目的:用培美曲塞(pemetrexed,PMX)建立叶酸缺乏的神经管畸形(neural tube defect,NTD)动物模型,并探讨其对神经上皮细胞增殖和凋亡的影响。方法:应用不同剂量PMX(5、10、15、17.5、20和30 mg/kg)对C57BL/6孕鼠进行干预,筛选最佳造模剂量。采用磷酸化组蛋白H3(pH3免疫组化的方法以及TUNEL方法)观察胎鼠神经上皮增殖和凋亡情况。结果:随着PMX剂量的增加,小鼠胚胎的吸收胎和畸形率逐渐增加,其中17.5mg/kg体重组的NTD的发生率最高(30.6%),被筛选为最佳造模剂量。pH3免疫组化和TUNEL结果显示,与正常对照组相比,NTD小鼠胚胎神经上皮细胞增殖减少(P<0.05),凋亡增加(P<0.05)。结论:PMX能诱导NTD的发生,其机制可能是因为PMX阻断了叶酸代谢通路并导致神经上皮细胞的增殖抑制和过度凋亡。
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| 关 键 词： | 培美曲塞 神经管畸形 细胞增殖 细胞凋亡 小鼠 |
Effect of PMX on the development of neural tube defects and neuroepithelial cell proliferation and apoptosis |
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| Guan Tao,Wang Xiuwei,Guan Zhen,Wang Jianhua,Wang Guoliang,Zhu Zhiqiang,Xie Qiu,Niu Bo. Effect of PMX on the development of neural tube defects and neuroepithelial cell proliferation and apoptosis[J]. Chinese Journal of Neuroanatomy, 2011, 27(4): 447-452 |
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| Authors: | Guan Tao Wang Xiuwei Guan Zhen Wang Jianhua Wang Guoliang Zhu Zhiqiang Xie Qiu Niu Bo |
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| Affiliation: | Guan Tao1,Wang Xiuwei2,Guan Zhen2,Wang Jianhua2,Wang Guoliang2,Zhu Zhiqiang2,Xie Qiu2,Niu Bo2(1.Department of Biochemistry and Molecular Biology,Shanxi Medical University,Taiyuan 030001,2.Capital Institute of Pediatrics,Beijing 100020,China) |
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| Abstract: | Objective: To establish neural tube defect(NTD) animal model by pemetrexed(PMX) and explore the effects of PMX on neuroepithelial cell proliferation and apoptosis.Methods: To select an optimal dose,C57BL/6 pregnant mice were injected intraperitoneally with different doses of PMX(5,10,15,17.5,20,and 30 mg/kg body weight).TUNEL and immunohistochemisty method for phosphorylated histone H3(pH3) were used to detect neuroepithelial cell proliferation and apoptosis of NTD embryos.Results: With the increasing of PM... |
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| Keywords: | pemetrexed neural tube defect cellular proliferation cellular apoptosis mice |
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