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| 细胞共培养条件下大鼠肌源性干细胞对氧化应激损伤神经元的保护作用 | |
| 引用本文： | 梅晰凡,刘世琼,刘畅,董娜,袁亚江,郭占鹏,李全双. 细胞共培养条件下大鼠肌源性干细胞对氧化应激损伤神经元的保护作用[J]. 神经解剖学杂志, 2011, 27(6): 658-664 |
| 作者姓名： | 梅晰凡 刘世琼 刘畅 董娜 袁亚江 郭占鹏 李全双 |
| 作者单位： | 1. 辽宁医学院附属第一医院骨科,,锦州121001 2. 河南科技大学,附属第一医院骨科,洛阳471003 3. 辽宁医学院附属第一医院内分泌科,锦州,121001 4. 河南科技大学附属第一医院病理科,洛阳,471003 |
| 基金项目： | 辽宁省自然科学基金(20072204); 辽宁省创新团队项目基金(2008T113) |
| 摘 要： | 目的:研究肌源性干细胞(MDSCs)与氧化应激损伤神经元共培养条件下MDSCs对后者的保护作用。方法:分离新生SD大鼠的MDSCs及皮层神经元,以叔丁基过氧化氢损伤神经元,构建氧化应激损伤的神经元模型。实验分为2组,损伤组:将第5代MDSCs和损伤的神经元置于共培养系统中培养4 h;对照组:损伤神经元于共培养系统中单独培养4 h。培养7 d后通过Hoster33258及流式细胞仪检测第2、4、6 d各组神经元的存活率,并用RT-PCR对相关基因进行分析,探究差异的内在原因。结果:Hoster33258及流式细胞仪检测结果显示:在共培养2、4、6 d三个时间点损伤组的神经元的存活率要远远高于对照组(P<0.05),凋亡率明显低于对照组,RT-PCR检测到损伤组神经元在三个时间点Bcl-2的表达水平均较对照组强(P<0.05),而Bax的表达水平均较对照组低(P<0.05)。结论:在共培养条件下,MDSCS对氧化应激损伤神经元具有保护作用,而这与其可促使损伤神经元Bcl-2的表达水平升高以及抑制Bax的表达有关。 |
| 关 键 词： | 肌源性干细胞 细胞培养 Bcl-2 Bax 大鼠 |
The protective effects of rats muscle-derived stem cells on neurons damage induced by oxidativestress in a co-culture system |
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| Mei Xifan , Liu Shiqong , Liu Chang , Dong Na , Yuan Yajiang , Guo Zhanpeng , Li Quanshuang. The protective effects of rats muscle-derived stem cells on neurons damage induced by oxidativestress in a co-culture system[J]. Chinese Journal of Neuroanatomy, 2011, 27(6): 658-664 | |
| Authors: | Mei Xifan Liu Shiqong Liu Chang Dong Na Yuan Yajiang Guo Zhanpeng Li Quanshuang |
| Affiliation: | Mei Xifan1,Liu Shiqong2,Liu Chang3,Dong Na4,Yuan Yajiang1,Guo Zhanpeng1,Li Quanshuang1 (1.Department of Orthopaedics,3.Department of Endocrine,First Affiliated Hospital,Liaoning Medical University,Jinzhou 121001,2.Department of Orthopaedics,4.Department of Pathology,Henan University of Science and Technology,Luoyang 471003,China) |
| Abstract: | Objective: To study the protedive effects of muscle-derived stem cells(MDSCs) on neuronal damage induced by oxidative stress in a co-culture system.Methods: MDSCs and cortical neurons were isolated from newborn SD rats,oxidative stress neuronal damage model was induced by tert-butyl hydroperoxide in vitro.The co-cultured cells were divided into two groups randomly.Injury group: tert-butyl hydroperoxide treated group,the MDSCs of passage 5 were co-cultured with neurons which were treated with tert-butyl hydr... |
| Keywords: | muscle derived stem cells cellular culture Bcl-2 Bax rat |
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