| PAR4在背根神经节感觉神经元的表达及与TRPV1和CGRP的共存 |
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| 引用本文： | 陈丹,张在峰,王昭金.PAR4在背根神经节感觉神经元的表达及与TRPV1和CGRP的共存[J].神经解剖学杂志,2011,27(5):493-496. |
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| 作者姓名： | 陈丹 张在峰 王昭金 |
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| 作者单位： | 泰山医学院人体解剖学教研室,泰安,271000 |
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| 基金项目： | 国家自然科学基金(81070898); 山东省自然科学基金(ZR2009CM033) |
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| 摘 要： | 目的:研究蛋白酶活化受体4(PAR4)在小鼠背根神经节(DRG)感觉神经元的表达,及与瞬时受体电位香草酸亚型1(TRPV1)和降钙素基因相关肽(CGRP)的共存,为探讨PAR4在感觉神经伤害性刺激信号传导中的作用提供形态学依据。方法:免疫荧光组织化学双标方法结合激光共聚焦显微镜技术。结果:DRG内大量的感觉神经元表达PAR4。PAR4阳性胞体多为中、小型神经元,并可见部分阳性神经纤维。免疫荧光双标显示许多PAR4阳性神经元表达TRPV1或与CGRP共存。几乎所有的TRPV1阳性神经元均表达PAR4,大部分CGRP标记神经元呈PAR4阳性,另外还可见到许多CGRP/TRPV1双标神经元。结论:小鼠DRG初级感觉神经元广泛的表达PAR4,该受体可能通过影响TRPV1和CGRP参与伤害性刺激的调节。
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| 关 键 词： | 蛋白酶活化受体4 瞬时受体电位香草酸亚型1 降钙素基因相关肽 背根神经节 免疫荧光组织化学 小鼠 |
Expression of PAR4 and its co-localization with TRPV1 and CGRP in sensory neurons of the dorsal root ganglia |
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| Chen Dan,Zhang Zaifeng,Wang Zhaojin.Expression of PAR4 and its co-localization with TRPV1 and CGRP in sensory neurons of the dorsal root ganglia[J].Chinese Journal of Neuroanatomy,2011,27(5):493-496. |
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| Authors: | Chen Dan Zhang Zaifeng Wang Zhaojin |
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| Institution: | Chen Dan,Zhang Zaifeng,Wang Zhaojin (Department of Anatomy,Taishan Medical University,Taian 271000,China) |
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| Abstract: | Objective: To study the expression of the protease-activated receptor-4(PAR4) and its co-localization with transient receptor potential vanilloid type 1(TRPV1) or calcitonin gene-related peptide(CGRP) in the sensory neurons of the mouse dorsal root ganglia(DRG),and provide the morphological evidence for exploring the role of PAR4 in the signal transduction of nociceptive stimuli in primary afferents.Methods: Double labeling fluorescent immunohistochemistry combined with laser scanning confocal microscope te... |
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| Keywords: | protease-activated receptor-4 transient receptor potential vanilloid type 1 calcitonin gene-related peptide dorsal root ganglion fluorescent immunohistochemistry mouse |
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