| 纳米二氧化锰对大鼠腹侧中脑的损伤作用 |
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| 引用本文： | 史婷婷,李涛,晋光荣,王健华,刘袁静,陈哲,朱建宝. 纳米二氧化锰对大鼠腹侧中脑的损伤作用[J]. 神经解剖学杂志, 2011, 27(6): 623-627 |
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| 作者姓名： | 史婷婷 李涛 晋光荣 王健华 刘袁静 陈哲 朱建宝 |
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| 作者单位： | 东南大学医学院人体解剖与组织胚胎学系,南京,210009 |
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| 基金项目： | 环境医学工程教育部重点实验室开放课题基金(2010EME008) |
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| 摘 要： | 目的:探讨纳米二氧化锰(nano-MnO2)对大鼠腹侧中脑的损伤作用。方法:大鼠在脑立体定位下,实验组脑内注射nano-MnO2,对照组脑内注射生理盐水(NS),各组分别于注射1周后用免疫组织化学方法检测酪氨酸羟化酶(tyrosine hydroxylase,TH)、胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)及诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)的表达变化。结果:免疫组织化学染色结果显示,实验组大鼠注射1周后,腹侧中脑损毁侧与对侧相比,TH阳性细胞明显减少(P<0.05),GFAP及iNOS阳性细胞明显增多(P<0.05);对照组大鼠注射1周后,腹侧中脑损毁侧与对侧相比,TH、GFAP和iNOS免疫反应阳性细胞均无明显变化(P>0.05)。结论:脑内注射nano-MnO2能引起大鼠中脑多巴胺能神经元的破坏,GFAP和iNOS的表达增加。
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| 关 键 词： | 纳米二氧化锰 多巴胺能神经元 胶质纤维酸性蛋白 诱导型一氧化氮合酶 |
The injury effect of nano-MnO_2 on the ventral midbrain of rats |
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| Shi Tingting , Li Tao , Jin Guangrong , Wang Jianhua , Liu Yuanjing , Chen Zhe , Zhu Jianbao. The injury effect of nano-MnO_2 on the ventral midbrain of rats[J]. Chinese Journal of Neuroanatomy, 2011, 27(6): 623-627 |
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| Authors: | Shi Tingting Li Tao Jin Guangrong Wang Jianhua Liu Yuanjing Chen Zhe Zhu Jianbao |
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| Affiliation: | Shi Tingting,Li Tao,Jin Guangrong,Wang Jianhua,Liu Yuanjing,Chen Zhe,Zhu Jianbao (Department of Anatomy,Histology and Embryology,School of Medicine of Southeast University,Nanjing 210009,China) |
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| Abstract: | Objective: To observe the injury effect of nano-MnO2 on the ventral midbrain of rats.Methods: Nano-MnO2 or 0.9% saline was stereotactically injected into the ventral midbrain of nano-MnO2-treated group and saline-treated group rats.After being injured 1week,the expression of tyrosine hydroxylase(TH),glial fibrillary acidic protein(GFAP) as well as inducible nitric oxide synthase(iNOS)were detected by immunohistochemical staining.Results: After 1 week of injection,there was a significant decrease of TH posit... |
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| Keywords: | nano-manganese dioxide dopaminergic neurons glial fibrillary acidic protein inducible nitric oxide synthase |
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