| EB病毒膜抗原BLLF1基因在转基因小鼠中的表达 |
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| 引用本文： | 郭长占,韩文龙,潘秀芳,周为民,谷淑燕,田枫,刘斌,高晓明. EB病毒膜抗原BLLF1基因在转基因小鼠中的表达[J]. 北京大学学报(医学版), 2000, 32(4): 326-327 |
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| 作者姓名： | 郭长占 韩文龙 潘秀芳 周为民 谷淑燕 田枫 刘斌 高晓明 |
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| 作者单位： | ^A北京大学基础医学院免疫学系,北京,100083^B北京大学^C1447^D1%^A北京大学人体解剖学与组织胚胎学系,北京,100083^B北京大学^C1447^D2%^A中国预防医学科学院病毒学研究所^B中国预防医学科学院^C26362^D3%^A北京大学实验动物科学部,北京,100083^B北京大学^C1447^D4 |
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| 摘 要： | 目的:分析EB病毒膜抗原(membrane antigen, MA)BLLF1基因在转基因小鼠外周血淋巴细胞中的表达.方法:EB病毒膜抗原BLLF1基因转基因首建鼠4只,尾组织经PCR检测携带有BLLF1基因,用流式细胞仪分析MA在外周血淋巴细胞中的表达并用激光共焦显微镜确定MA在细胞中的表达部位.结果:4只首建鼠中有2只KM-Tg-EBV1和KM-Tg-EBV5外周血淋巴细胞中有MA的表达,表达部位在细胞浆中和细胞膜上.结论:表达EB病毒膜抗原的转基因小鼠可作为研究EB病毒致病机制的一个新的动物模型.
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| 关 键 词： | 抗原 病毒 基因表达 小鼠 转基因 基因 BLLF1 淋巴细胞 |
Expression of Epstein-Barr virus BLLF1 gene in transgenic mice |
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| GUO Chang-Zhan,HAN Wen-Long,PAN Xiu-Fang,ZHOU Wei-Min,GU Shu-Yan,TIAN Feng,LIU Bin,GAO Xiao-Ming. Expression of Epstein-Barr virus BLLF1 gene in transgenic mice[J]. Journal of Peking University. Health sciences, 2000, 32(4): 326-327 |
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| Authors: | GUO Chang-Zhan HAN Wen-Long PAN Xiu-Fang ZHOU Wei-Min GU Shu-Yan TIAN Feng LIU Bin GAO Xiao-Ming |
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| Affiliation: | GUO Chang Zhan 1,HAN Wen Long 1,PAN Xiu Fang 2,ZHOU Wei Min 4,GU Shu Yan 4,TIAN Feng 3,LIU Bin 2,GAO Xiao Ming 1 |
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| Abstract: | Objective: To analyze the expression of Epstein Barr virus (EBV) BLLF1 gene expression in peripheral lymphocytes of transgenic founder mice. Methods: BLLF1 expression in peripheral lymphocytes was analyzed by flow cytometry and the localization of expressed products in the lymphocytes was determined by confocal microscopy. Results: BLLF1 gene expression was detectable in two of the four transgenic founders. The expression products localise in the cytoplasm and on the surface of lymphocytes. Conclusion: Transgenic mice harboring EBV BLLF1 gene could be used as a model in pathogenesis of EBV. |
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| Keywords: | Herpesvirus 4 human Antigens viral Gene expression Mice transgenic Gene BLLF1 Lymphocytes |
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