海昆肾喜对阿霉素肾病大鼠肾组织结缔组织生长因子蛋白及其mRNA表达的影响

目的:探讨海昆肾喜对阿霉素肾病大鼠肾组织结缔组织生长因子(connective tissue growth factor,CTGF)蛋白及其mRNA的影响.方法:将54只大鼠随机分为6组,正常组、假手术组、模型组、苯那普利组和海昆肾喜小剂量组与大剂量组.采用单侧肾切除阿霉素肾病模型.术后10周处死大鼠,观察肾组织病理改变,并应用免疫组织化学方法和原位杂交法检测肾组织CTGF及其mRNA的表达.结果:正常组及假手术组肾小管上皮细胞、肾间质细胞胞浆内有少量CTGF及其mRNA阳性反应物;模型组肾小管上皮细胞、肾间质细胞内的CTGF及其mRNA在细胞浆内呈强阳性表达;海昆肾喜小剂量组、大剂量组表达较模型组显著减少(P＜0.05).结论:海昆肾喜可抑制CTGF蛋白及其mRNA的过度表达,进而减缓肾纤维化的病程进展.

The Effect of Haikunshenxi Caps on the Connective Tissue Growth Factor Protein and its mRNA''''s Expression on Adriamycin-induced Nephropathicy Rats

Objective: To investigate the change of connective tissue factor (CTGF) in adriamycin-induced nephropathy rats, and study the effect of Haikunshenxi on adrimysin-induced nephropathy rats and its possible mechanism.Methods:Half nephrectomize with ADR nephropathy was used. The rats were randomly divided into 6 groups: normal control group, adriamysin-induced nephropathy group, sham operation group, Lotensin treated group and Haikunshenxi low dose and big dose treatment group. Renal tissue were examined by light microscopy at 10 weeks after operation.Immunohistochemistry and in-situ hybridization was applied to measure the expression of CTGF and CTGF mRNA in the nephridial tissue.Results:The expression of CTGF and CTGF mRNA in the nomal and sham operation group is low in the intracytoplasm of Mesangial cells, renaltubular epithelial cells and renal tubular epithelial cells. Compare with sham operation group, the expression of CTGF and CTGF mRNA in the mode group was markedly increased, and the fibrosis of nephridial tissue is obvious. Haikunshenxi could significantly decrease the expression of CTGF and CTGF mRNA (P<0.05).Conclusion:Haikunshenxi may suppress the development of fibrosis, at least in part via down-regulating the expression of protein and mRNA of CTGF.