Survivin反义核酸对大肠癌HT-29细胞化疗的影响

目的:探讨反义寡核苷酸对大肠癌化学治疗敏感性的影响。方法:设计针对Survivin模板序列的经硫代磷酸修饰的反义和正义核苷酸链的寡核苷酸链,导入大肠癌HT-29细胞中,观察其对大肠癌细胞活力、细胞增殖能力的影响以及Survivin反义核酸对大肠癌细胞化疗敏感性的影响。结果:ASODN明显抑制大肠癌细胞的集落形成能力。低浓度5-FU Survivin反义核酸对HT-29大肠癌细胞的抑制作用明显高于单独低浓度5-FU组和低浓度5-FU SODN组,其差异有统计学意义,P<0.05。结论:Survivin反义寡核苷酸作用后的大肠癌HT-29细胞株生长受到抑制,Survivin反义寡核苷酸对体外培养的大肠癌细胞化疗有增敏效应。

Survivin antisense oligodeoxynucleotides effect on the chemotherapy of HT-29 colorectal carcinoma cells

OBJECTIVE:To investigate the effect of Survivin gene on the growth of human colorectal cancer HT-29 cells and to explore the feasibility of gene therapy in human colorectal carcinoma.METHODS:The antisense oligodeoxynucleotides were complementary to Survivin sequences.The human colorectal cancer HT-29 cell line was treated with ASODNs.The effects of the Survivin-ASODN on the growth of HT-29 and apoptosis were studied by cell colony assay.Low concentration 5-FU was added to study the sensitivity of colorectal cancer cell induced by ASODNs to chemotherapy.RESULTS:The cell proliferation was inhibited effectively by antisense oligodeoxynucleotides to Survivin gene.Compared with the control group,the percentage of the survival cells significantly decreased in the presence of both 5-FU and Survivin ASODNs,P<0.05.CONCLUSIONS:The antisense oligodeoxynucleotides were capable of inhibiting the proliferation of human colorectal carcinoma HT-29 cells.Combination of Survivin ASODN and 5-FU might improve the treatment outcome of colorectal carcinoma.