Altered regulation of metabolic pathways in human lung cancer discerned by 13C stable isotope-resolved metabolomics (SIRM)期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Altered regulation of metabolic pathways in human lung cancer discerned by 13C stable isotope-resolved metabolomics (SIRM)

Authors:	Teresa WM Fan Andrew N Lane Richard M Higashi Mohamed A Farag Hong Gao Michael Bousamra Donald M Miller

Affiliation:	(1) Department of Chemistry, University of Louisville, Louisville, Kentucky 40292, USA;(2) James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky 40292, USA;(3) Department of Surgery, University of Louisville, Louisville, Kentucky 40292, USA;(4) Center for Regulatory and Environmental Analytical Metabolomics (CREAM), University of Louisville, Louisville, Kentucky 40292, USA;(5) Pharmacognosy Department, Faculty of Pharmacy, Kasr El Aini St, P.B. 11562, Cairo University, Egypt

Abstract:	Background Metabolic perturbations arising from malignant transformation have not been systematically characterized in human lung cancers in situ. Stable isotope resolved metabolomic analysis (SIRM) enables functional analysis of gene dysregulations in lung cancer. To this purpose, metabolic changes were investigated by infusing uniformly labeled ¹³C-glucose into human lung cancer patients, followed by resection and processing of paired non-cancerous lung and non small cell carcinoma tissues. NMR and GC-MS were used for ¹³C-isotopomer-based metabolomic analysis of the extracts of tissues and blood plasma.

Keywords:
本文献已被 SpringerLink 等数据库收录！