Immunohistochemical localization of macromolecules of the basement membrane and the peritumoral stroma in human laryngeal carcinomas

Forty laryngeal carcinomas were studied by immunofluorescence with specific antisera against components of the basement membrane (type IV collagen and laminin) as well as antisera against connective tissue antigens (type V collagen and fibronectin). The basement membrane surrounding well-differentiated squamous cell carcinomas showed an appearance similar to that seen beneath normal epithelium. In contrast, marked alterations of the basement membrane were constantly observed around infiltrating and poorly-differentiated carcinomas. The staining of connective tissue components in most cases was as intense in carcinomas as in normal laryngeal mucosa. The use of antibodies to basement membrane components may help to elucidate the mechanism of invasion of connective tissues by malignant cells.