内镜消毒机与配套的固体过氧乙酸杀灭微生物效果的实验观察

为观察内镜消毒机及其配套的固体过氧乙酸对微生物的杀灭效果,采用载体浸泡定量法和模拟现场试验对其消毒效果进行了观察。结果,以含58.95 mg/L过氧乙酸和386.30 mg/L过氧化氢的固体过氧乙酸消毒剂溶液,对不锈钢片上的金黄色葡萄球菌、大肠杆菌作用7 m in杀灭率均达100%;以含过氧乙酸1179 mg/L、过氧化氢7726mg/L的该消毒液作用10 m in,对枯草杆菌黑色变种芽孢的杀灭率达100%。模拟现场试验中,将含过氧乙酸1179mg/L、过氧化氢7726 mg/L固体过氧乙酸消毒剂置于专门内窥镜消毒机内,按机械规定程序进行循环消毒10 m in、无菌水冲洗和干燥等处理程序共20 m in,对污染在模拟载体及胃镜、支气管镜内腔的枯草杆菌黑色变种芽孢的消除率≥99.99%。结论,该内窥镜消毒机与其专用固体过氧乙酸水解化合生成过氧乙酸、过氧化氢进行消毒,具有良好的消毒效果。

EXPERIMENTAL OBSERVATION ON GERMICIDAL EFFICACY OF ENDOSCOPE DISINFECTOR AND ITS ACCOMPANYING SOLID PERACETIC ACID

In order to observe the germicidal efficacy of endoscope disinfector and accompanting solid peracetic acid,carrier immersion quantitative method and simulated field trial were used to observe its disinfection efficacy.Results: The solid peracetic acid disinfectant solution containing peracetic acid 58.95% and hydrogen peroxide 386.30 mg/L with a 7 min contact time killed 100% of both Staphylococcus aureus and Escherichia coli on stainless steel coupons and the disinfectant solution containing peracetic acid 1179 mg/L and hydrogem peroxide 7726 mg/L with a 10 min contact time killed 100% of spores of Bacillus subtilis var.niger.In the simulated field trial,after adding the solid peracetic acid disinfectant containing peracetic acid 1179 mg/L and hydrogen peroxide 7726 mg/L to the special endoscope disinfector,operation of the disinfector according to the specified 20 min program consisting of circulating disinfection for 10 min,sterile water flushing and drying eliminated ≥(99.99%) of the spores of Bacillus subtilis var.niger on simulated carriers and in the lumens of gastroscope and bronchoscope.Conclusion: Disinfection with the endoscope disinfector and peracetic acid and hydrogen peroxide produced by hydrolysis of the special-purpose solid peracetic acid of the disinfector has good efficacy.