SWA-Fab''''对肿瘤细胞的凋亡作用

目的研究SEA-Fab'对肿瘤细胞的凋亡作用及单克隆抗体于肿瘤定向治疗作用.方法实验分为三组SEA-Fab'、SEA及对照组,三组肿瘤细胞分别用SEA-Fab'、SEA及PBMC+Walker-256细胞处理,在24～72h内观察肿瘤细胞的凋亡情况.结果SEA-Fab'组、SEA组的肿瘤细胞指数分别为(34.6%～68.9%)和(15.5%～31.9%)高于对照组(5.5%～12.5%),差异存在显著性,SEA-Fab'组高于SEA组差异显著.结论SEA-Fab'在激活T细胞和杀死肿瘤细胞效果好于SEA,将来有可能利用单克隆抗体来作定向靶位治疗.

Apoptosis function on tumor cell by SEA-Fab'''' coupled protein

[Objective] To Study the anti-tumor effect of the SEA-Fab' coupled protein and the possibility of themonoclonal antibody Fab's targeted in immunotherapy of human tumor. [Methods] The experiment group was treat-ed by SEA-Fab' and SEA, the contrast group was treated by PBMC+Walker-256 cell. The apoptotic index was ob-served in 24～72 h. [Results] The poptotic index was significantly higher in the SEA-Fab's (34.6～68.9%) and SEAgroups (15.5～31.9%) compared with the PBMC+Wallker-256 cell group (5.5～12.8%). There was significant differ-ence between SEA-Fab group and SEA group (P＜0.01). [Conclusion] SEA-Fab' is more effective to activate Tcells and kill tumor cell than SEA, there is ossibility of The monoclonal antibody targeted in immunotherapy of hu-man tumor.