VEGF、COX-2、mPGES mRNA在结肠癌中的表达及其意义

 目的 通过研究血管内皮生长因子（VEGF）、COX-2 和膜相关前列腺素E合成酶（mPGES）在结肠癌中的表达，探讨VEGF、COX-2 和mPGES与结肠癌的关系。方法 采用实时荧光定量RT-PCR法，对32例结肠癌组织及其相应癌旁组织中VEGF、COX-2 和mPGES mRNA的表达进行了研究。结果 32例癌组织中，VEGF121表达无变化。VEGF165高表达19例（59.3 %）；COX-2高表达20例（62.5 %）；mPGES高表达24例（75 %）。32例癌旁组织中，VEGF高表达6例（18.7 %）；COX-2高表达5例（15.6 %）；mPGES高表达9例（28.12 %）。结论 VEGF165、COX-2和mPGES与结肠癌的发生相关。COX-2和mPGES在结肠癌组织中上调程度存在差异，提示COX-2和mPGES这两种诱导型酶在调节机制上可能存在差异。

The expression of VEGF,COX2 and mPGES mRNA in colon cancer

Objective To study the expression of VEGF, Cox2 and mPGES in colon cancer. Methods VEGF, Cox2 and mPGES mRNA expression in 32 paired samples (tumor and adjacent normal tissue) were de－termined by using real time RT-PCR. Results VEGF was overexpressed in 19 of 32 (59.3 %) tumor tissues compared with that in 6 of 32 (18.7 %) adjacent normal tissue; COX2 was overexpressed in 20 of 32 (62.5 %) tumor tissues compared with that in 5 of 32 (15.6 %) adjacent normal tissue ;mPGES was overexpressed in 24 of 32 (75 %) tumor tissues compared with that in 9 of 32 (28.12 %) adjacent normal tissue. Conclusion Our result suggested that VEGF165, mPGES and COX2 overexpressed in colon cancer.