实时荧光定量RT-PCR检测良性前列腺增生与前列腺癌相关基因表达的临床意义

目的应用实时荧光定量RT-PCR检测良性前列腺增生（BPH）与前列腺癌相关基因表达。方法通过实时荧光定量RT-PCR对9个前列腺癌相关基因（KLK3、KLK2、KLK11，pim-1，hepsin，PSMA，AR，p27，IGF-1）在前列腺正常组织、BPH组织和癌组织（PCa）定量表达，分析其特异性的差异。结果检测9个选定相关基因中发现KLK2，pim-1，AR和IGF-1在这3种组织中的表达差异有统计学意义（P＜0．01）。结论KLK2，pim-1，AR和IGF-1作为前列腺癌组织特异性的分子标记物组合，有望提高前列腺癌早期诊断的准确性。

Study on the expression of BPH and prostate cancer related genes using real-time quantitative RT-PCR

Objective To determine the expression of pr ostate hyperplasia- and cancer-related genes by real-time quantitative RT-PC R and to screen for those which can be used for clinical diagnosis of prostate c ancer. Methods Using real-time quantitative RT-PCR,the expression of 9 genes including KLK3,KLK2,KLK11,pim-1,hepsin,PSMA,AR,p27,IG F-1 in 3 prostate tissues,ie,normal tissue,BPH tissue and prostate cancer tissu e,were quantitated and their specifically differential expressions were analyzed . Results Of the 9 genes tested,4 genes,ie,KLK2, pim-1, AR and IGF-1,were differentially expressed in the 3 tissues.The differences wer e statistically significant (P<0.01). Conclusions Th e combination of KLK2,pim-1,AR and IGF-1 which are specific molecular markers of prostate cancer tissue may improve the rate of early diagnosis of prostate ca ncer.