| NS-398对肝细胞癌VEGF、MMP-9表达的调节 |
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| 引用本文： | 彭利,徐卓,张萌,王顺祥,唐瑞峰,何宏涛,左连富.NS-398对肝细胞癌VEGF、MMP-9表达的调节[J].肿瘤防治研究,2006,33(12):859-861. |
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| 作者姓名： | 彭利 徐卓 张萌 王顺祥 唐瑞峰 何宏涛 左连富 |
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| 作者单位： | 河北医科大学第四医院肝胆外科; 河北省肿瘤研究所; |
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| 摘 要： | 目的探讨NS-398对肝细胞癌血管生成和侵袭的调节作用。方法NS-398取0、25、50、75、100μmol/L5个浓度组,每个浓度组选取24h、48h、72h三个作用时间点进行检测。采用RT-PCR和流式细胞技术,观察NS-398对人肝癌SMMC-7721细胞株VEGF、MMP-9表达的影响。结果NS-398对SMMC-7721细胞有明显的生长抑制作用。与对照组相比,NS-398能显著抑制SMMC-7721细胞COX-2、VEGF、MMP-9基因和蛋白表达,并呈显著的剂量和时间依赖性关系(P<0.001)。结论NS-398能显著抑制SMMC-7721肝细胞癌中VEGF、MMP-9mRNA和蛋白的表达。
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| 关 键 词： | 肝细胞癌 NS-398 血管内皮生长因子 MMP-9 |
| 文章编号： | 1000-8578（2006）12-0859-03 |
| 收稿时间： | 2005-10-19 |
| 修稿时间： | 2005-10-192006-01-05 |
Regulation of NS-398 on Expressions of VEGF and MMP-9 in Hepatocullar Carcinoma SMMC-7721 Cell Lines |
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| PENG Li,XU Zhuo,ZHANG Meng,WANG Shun-xiang,TANG Rui-feng,HE Hong-tao,ZUO Lian-fu.Regulation of NS-398 on Expressions of VEGF and MMP-9 in Hepatocullar Carcinoma SMMC-7721 Cell Lines[J].Cancer Research on Prevention and Treatment,2006,33(12):859-861. |
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| Authors: | PENG Li XU Zhuo ZHANG Meng WANG Shun-xiang TANG Rui-feng HE Hong-tao ZUO Lian-fu |
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| Institution: | 1.Department of Hepatobiliary Surgery; Fourth Hospital of Hebei Medical University; Shijiazhuang 050011; 2.Hebei Cancer Institute; |
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| Abstract: | Objective To explore the role of NS-398 in angiogenesis and invasion in hepatocellular carcinoma. Methods For dose-dependent experiments, media containing various concentrations of NS-398 (0, 25, 50, 75 and 100μmol/L) were used. For time course experiments, media containing various concentrations of NS-398 were used, and the cells were harvested daily for 3 days. The expression of VEGF and MMP-9 in SMMC-7721 cells were determined by RT-PCR and flow cytometry method by the treatment with NS-398. Results NS-3... |
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| Keywords: | NS-398 MMP-9 |
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