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| 慢性阻塞性肺疾病患者诱导痰上清液及血清中基质金属蛋白酶的表达 | |
| 引用本文： | 郭瑞霞,张建敏,王佳玲,吴金芳,袁雅冬. 慢性阻塞性肺疾病患者诱导痰上清液及血清中基质金属蛋白酶的表达[J]. 临床荟萃, 2011, 26(11): 930-932 |
| 作者姓名： | 郭瑞霞 张建敏 王佳玲 吴金芳 袁雅冬 |
| 作者单位： | 1. 邯郸市第一医院,呼吸内科,河北,邯郸,056002 2. 邯郸市第一医院,检验科,河北,邯郸,056002 3. 河北医科大学第二医院呼吸内科,河北,石家庄,050000 |
| 摘 要： | 目的检测慢性阻塞性肺疾病（COPD）不同时期患者诱导痰上清液及血清中基质金属蛋白酶9（MMP-9）、基质金属蛋白酶组织抑制剂1（TI MP-1）浓度,分析其与COPD病情变化的关系。方法选择COPD缓解期组、COPD急性加重期（AECOPD）组和正常对照组各20例,采用双抗夹心酶联免疫吸附测定法检测各组受试者诱导痰上清液及血清中MMP-9、TI MP-1的浓度。结果AECOPD组和COPD缓解期组诱导痰上清液及血清MMP-9浓度均高于正常对照组,诱导痰上清液MMP-9（612.2±58.9）μg/L、（401.5±45.7）μg/L vs（186.2±77.4）μg/L（P〈0.01）,血清MMP-9（221.7±30.4）μg/L、（203.0±33.5）μg/Lvs（131.4±44.5）μg/L（P〈0.01）;AECOPD组诱导痰上清液及血清MMP-9浓度均高于COPD缓解期组（P〈0.01）。AECOPD组和COPD缓解期组诱导痰上清液及血清TI MP-1浓度均高于正常对照组,诱导痰上清液TI MP-1（859.3±101.0）μg/L、（356.4±92.1）μg/L vs（97.1±54.2）μg/L（P〈0.01）,血清TI MP-1（213.2±27.2）μg/L、（149.9±20.1）μg/L vs（84.1±14.0）μg/L（P〈0.01）;AECOPD组血清TI MP-1浓度均高于COPD缓解期组（P〈0.01）。AECOPD组和COPD缓解期组诱导痰上清液及血清MMP-9/TI MP-1比值均低于正常对照组（P〈0.01）;AECOPD组血清MMP-9/TI MP-1比值低于COPD缓解期组（P〈0.01）。结论COPD不同时期诱导痰及血清中MMP-9、TI MP-1含量不同,提示存在蛋白酶/抗蛋白酶失衡机制。 |
| 关 键 词： | 肺疾病,慢性阻塞性 基质金属蛋白酶9 痰 血清 |
Expression of matrix metalloproteinase in induced sputum and serum in patients with chronic obstructive pulmonary disease |
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| GUO Rui-xia,ZHANG Jian-min,WANG Jia-ling,WU Jin-fang,YUAN Ya-dong. Expression of matrix metalloproteinase in induced sputum and serum in patients with chronic obstructive pulmonary disease[J]. Clinical Focus, 2011, 26(11): 930-932 | |
| Authors: | GUO Rui-xia ZHANG Jian-min WANG Jia-ling WU Jin-fang YUAN Ya-dong |
| Affiliation: | GUO Rui-xia1a,ZHANG Jian-min1b,WANG Jia-ling1b,WU Jin-fang1a,YUAN Ya-dong21a.Department of Respiration,1b.Department of Clinical Laboratory,the First Hospital of Handan,Handan 056002,China,2.Department of Respiration,the Second Hospital of Hebei Medical University,Shijiazhuang 050000 |
| Abstract: | Objective To investigate the levels of matrix metalloproteinase 9(MMP-9) and tissue inhibitors of matrix metalloproteinases 1(TIMP-1) in induced sputum and serum in patients with chronic obstructive pulmonary disease(COPD) during different periods,and its relationship with the state of illness.Methods Enzyme-linked immunosorbent assay(ELISA) was used to test the levels of MMP-9,TIMP-1 in induced sputum and serum in 20 normal persons,20 patients with COPD during an acute exacerbation(AECOPD) and 20 patients ... |
| Keywords: | pulmonary disease chronic obstructive matrix metalloproteinase 9 sputum serum |
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