Antibodies Reactive to Trypanosoma cruzi Epimastigotes or Amastigotes Express Different Idiotypic Patterns if from Patients with Different Clinical Forms of Chagas' Disease |
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| Authors: | R. RIBEIRO-RODRIGUES,D. G. COLLEY,R. CORREA-OLIVEIRA,& C. E. CARTER |
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| Affiliation: | Department of Biology, Vanderbilt University, Nashville, TN;, Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, GA;, Centro de Pesquisas RenéRachou–FIOCRUZ, Belo Horizonte, Brazil |
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| Abstract: | Antibodies (Abs) were purified from pooled sera of patients with either indeterminate (IND or I) or cardiac (CARD or C) Chagas' disease, on either epimastigote (EPI or E) or amastigote-enriched (AMAST or A) antigen (Ag) columns and their idiotypic (Id) expression examined. Anti-Id rabbit Abs were raised to the different preparations (E-IdI, E-IdC, A-IdI and A-IdC). Competitive ELISAs using anti-Ids were able to discriminate between IdI and IdC, disregarding Ag reactivity. E-IdI and A-IdI present different inhibitory abilities, as do E-IdC and A-IdC, but IdC always competes with IdI for anti-IdI comparably. In contrast, a 4–8-fold increase of IdI is required to compete in parallel with IdC for anti-IdC. Therefore, Ids from IND patients share only low levels of the Ids that are most characteristic of CARD patients. While some CARD Abs also express Ids in common with IND patients, these studies reveal that CARD Abs express some Ids that are characteristic to only CARD patients, and these Ids are present on Abs purified with either EPI or AMAST. |
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