白细胞介素17在原发性肝癌中的表达及意义

目的:对白细胞介素17(IL-17)在原发性肝癌组织中的表达及其意义进行探讨。方法:用酶联免疫吸附试验测定本院2011年5月-2012年5月的术前与术后165例原发性肝癌患者、35例肝良性肿瘤患者及30例健康对照者血清中的IL-17水平,对手术切除的肝癌组织、相应癌旁组织标本行实时荧光定量聚合酶链反应检测IL-17表达水平。结果:原发性肝癌组术前与术后血清IL-17表达水平明显高于良性肿瘤组与健康对照组,差异有显著性(P<0.01)。肝癌患者癌及癌旁组织中的IL-17的相对表达量高于良性肿瘤组,差异有统计学意义(P<0.05)。肝癌患者癌组织中IL-17的相对表达量高于其癌旁组织,差异有统计学意义(P<0.05)。结论:IL-17表达升高与原发性肝癌密切相关,对临床诊疗可能发挥重要作用。

Expression and clinical significance of IL-17 in primary human hepatocellular carcinoma tissues

Objective:To investigate the expression and clinical significance of IL-17 in primary human hepatocellular carcinoma(HCC).Methods:Serum from 165 primary HCC patients and 35 benign liver tumor patients and 30 normal controls were collected for the present study.The serum IL-17 level was measured by ELISA,the expression of IL-17 mRNA in HCC tissues via surgical excision were detected by real-time PCR.Results:The serum IL-17 level was greatly higher in HCC patients compared with that in benign liver tumor patients and normal controls.Real-time PCR results showed that the expression level of IL-17 mRNA was significantly higher in cancer tissues than that in the peritumoral and normal liver tissues(P<0.05).Conclusion:IL-17 obviously increased in HCC tissues and serum,indicating that it may play an important role in the diagnosis and treatment of HCC.