| 乳腺癌患者血浆线粒体D环高变区基因突变研究 |
| |
| 引用本文： | 靳斌,任军,张亮,汪海丹,白俊,张利旺,斯小明. 乳腺癌患者血浆线粒体D环高变区基因突变研究[J]. 临床肿瘤学杂志, 2005, 10(1): 1-3,10 |
| |
| 作者姓名： | 靳斌 任军 张亮 汪海丹 白俊 张利旺 斯小明 |
| |
| 作者单位： | 710032,西安,第四军医大学西京医院肿瘤中心;710032,西安,第四军医大学西京医院肿瘤中心;710032,西安,第四军医大学西京医院肿瘤中心;710032,西安,第四军医大学西京医院肿瘤中心;710032,西安,第四军医大学西京医院肿瘤中心;710032,西安,第四军医大学西京医院肿瘤中心;710032,西安,第四军医大学西京医院肿瘤中心 |
| |
| 基金项目： | 全国高校优秀青年教师教学科研奖励计划(TRAPOYT99-16)，高等学校骨干教师资助计划(FUKT2000-06)项目 |
| |
| 摘 要： | 目的:研究检测血浆线粒体DNA基因突变对于乳腺癌诊断的参考价值。方法:选取乳腺癌患者35例,健康 对照者10人,提取血浆DNA,设计两对引物,采用聚合酶链反应(PCR)扩增血浆线粒体DNA的D环HVR1和HVR2,然后用 变性高效液相色谱法(DHPLC)对产物进行突变筛选。结果:乳腺癌患者的血浆DNA扩增率(46/140)和筛选的突变率(5/46) 与对照组比较,差异具有显著性(P<0.05)。结论:检测血浆线粒体DNA基因突变可能对于乳腺癌的诊断具有重要意义。
|
| 关 键 词： | 乳腺癌 线粒体DNA D环 高变区 血浆 |
| 文章编号： | 1009-0460(2005)01-0001-04 |
The research for mtDNA D-loop gene mutation in plasma of primary breast cancer patients |
| |
| JIN Bin,REN Jun,ZHANG Liang,et al.. The research for mtDNA D-loop gene mutation in plasma of primary breast cancer patients[J]. Chinese Clinical Oncology, 2005, 10(1): 1-3,10 |
| |
| Authors: | JIN Bin REN Jun ZHANG Liang et al. |
| |
| Affiliation: | JIN Bin,REN Jun,ZHANG Liang,et al.Cancer Center,Xijing Hospital,the Fourth Military Medical University of PLA,Xi'an 710032 |
| |
| Abstract: | Objective:This study was designed to identify mtDNA D-loop HVR gene mutations in plasma of primary breast cancer patients to study the clinical significance of mtDNA D-loop gene mutations associated with breast cancer.Methods:The plasma DNA in 35 primary breast cancer patients and 10 normal healthy people were extracted and mtDNA D-loop HVR1 and HVR2 fragments were amplified with designed two pairs of primers by PCR.The mutations of products were screened by denaturing high performance liquid chromatography(DHPLC).Results:Comparing with that of healthy group,amplified DNA ratio(46/140)and mutation ratio(5/46) in plasma of breast cancer have statistic means(P<0.05).Conclusion:It indicates that mtDNA gene mutations in plasma may play an important role in primary breast cancer carcinogenesis. |
| |
| Keywords: | Primary breast cancer mtDNA D-loop HVR Plasma |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
