谷氨酸单钠对大鼠骨发育的影响

观察出生１０ｄ内皮下注射ＭＳＧ的Ｗｉｓｔａｒ大鼠，新生期（１１日龄）、青春期（５２日龄）、成年期（１００日龄）长骨发育情况。结果表明：青春期及成年期上、下肢各长骨骨干长度、胫骨骺板厚度及骺板增殖区厚度，ＭＳＧ组均落后于对照组（Ｐ＜０．０５或０．０１），１１日龄时两组长骨骨干长度虽无显著性差异，但决定长骨生长速度的骺板增殖区厚度已出现了显著性差异。各年龄期ＭＳＧ组骺板结构及骨生长发育过程无明显异常，仅厚度变薄。有别于因维生素类、甲状旁腺素等因素引起的生长迟缓。结合各年龄期ＭＳＧ处理鼠垂体湿重分别为对照组的５５．５６％、４２．３９％和４７．７０％，ＭＳＧ处理鼠身长、体重均比对照鼠低２个标准差以下。认为骨的发育及骺板变化符合ＧＨ分泌减少所致。

Effect of Monosodium Glutamate on Bone Development in Rats

The evidence of dwarfism-like manifestation of rat was found by administration of hypodermic MSG 0. 4 g/kg to the new born one every other day within 10 days after birth.We observed the rat bone development on the 11 th day(new born stage),52 th day (puberty) and 100 th day(adult).The result showed that the trunk Jength of long bones of upper limb and lower limb,the thickness of tibia epiphysis and its multiplication area revealed signeficantly the development delay in the experimental groups P<0.05 for puberty groups,P<0.01 for the adule groups.For the 11 th day groups,the length of long bone was on difference,but the thickness of epiphysis multiplication area,which determined the bone growth rate,decreased obviously.We could not discover the difference on the histologicalstructure of epiphysis and the development course of bone between experimental group and control group.The weight of pituitary gland in the experimetal groups possess of 55. 6%(11 th day group),42.4 %(52 th day group)and 47.7 %(100 th day group)of control groups. The immunohistochemical result indicated the number of GH-secreting cell was decreased.Cell volume was smaller.The body length and weight of the rats experimental groups were below 2 S of control group.According to our results,the MSG could induce the rat dwasfism with GH deficiency.