HGC-1在胃癌中的表达及其与胃癌临床病理学因素和预后的关系

目的检测HGC-1在胃癌中的表达,探讨其与胃癌临床病理学因素和预后的关系。方法应用实时定量聚合酶链反应技术检测30例胃癌中HGC-1 mRNA的表达,免疫组织化学方法检测89例胃癌、正常黏膜及20例胃黏膜肠上皮化生组织中HGC-1蛋白的表达。结果 HGC-1 mRNA在73.3%的胃癌中高表达;HGC-1蛋白在胃癌及肠上皮化生组织中的表达率分别为68.5%和65%,均显著高于正常黏膜(7.9%,P<0.001),胃癌与肠上皮化生组织中HGC-1蛋白的表达无明显差异(P>0.05);HGC-1蛋白表达阳性与胃癌分化(P<0.01)、PCNA(P<0.05)相关。HGC-1蛋白阳性患者总体生存率高于阴性患者,HGC-1是影响总体生存的独立预后因素。结论HGC-1在胃癌发生中起重要作用,可能是早期诊断和预后评估的指标。

HGC-1 expression in relation with clinicopathological features and prognosis of gastric cancer

Objective To study the expression of HGC-1 in gastric cancer and its relation with clinicopathological features and prognosis.Methods HGC-1 mRNA expression was examined in 30 cases of gastric cancer and corresponding para-cancerous tissues by real-time polymerase chain reaction.HGC-1 protein expression was studied by immunohistochemistry,covering 89 samples of primary gastric cancer and corresponding para-cancerous tissues,along with 20 intestinal metaplasia tissues.Results HGC-1 mRNA was up-regulated more than 2-fold in 73.3% patients with gastric cancer.HGC-1 protein was up-regulated in gastric cancer,correlating with the differentiation status of gastric carcinoma and index.Immnoreactivity of HGC-1 was significantly higher in gastric cancer and intestinal metaplasia tissues than in normal mucosa.Patients positive for HGC-1 had higher overall survival rates(OS) than those negative for HGC-1.HGC-1 was an independent prognostic factor for OS(P<0.01).Conclusion HGC-1 may play an important role in gastric cancer tumorigenesis and could be used as an indicator for diagnosis and prognosis in gastric cancer.