RhoE/Rnd3蛋白在子宫腺肌病中的表达及意义

目的探讨RhoE/Rnd3蛋白在子宫腺肌病组织中的表达及其潜在的临床意义。方法应用免疫组织化学方法检测32例正常子宫(对照组)内膜、平滑肌组织及38例子宫腺肌病组织(实验组)中在位内膜(A组)、异位内膜(B组)、平滑肌组织中RhoE/Rnd3蛋白的组织定位和表达程度,分析对比其差异。结果①腺上皮细胞:RhoE/Rnd3蛋白在对照组腺体细胞中的表达均高于实验组在位内膜腺体细胞(t=11.3,P<0.05)及异位内膜腺体细胞(t=9.33,P<0.05);在子宫腺肌病在位内膜腺体细胞与异位内膜腺体细胞中的表达差异无统计学意义(t=1.63,P>0.05);②间质细胞及平滑肌:RhoE/Rnd3蛋白在3组内膜间质细胞及对照组-实验组平滑肌细胞中表达差异均无统计学意义(P>0.05);③子宫腺肌病内膜腺体细胞中RhoE/Rnd3蛋白的表达水平与患者的病程有关,病程超过2年的患者组织中,RhoE/Rnd3蛋白的表达水平明显低于病程不足2年的患者(P<0.05)。结论RhoE/Rnd3蛋白在子宫腺肌病的发生发展中发挥重要作用,有可能是一个重要的肿瘤抑制基因。

Expression and significance of RhoE/Rnd3 protein in adenomyosis

Objective To investigate expression of RhoE/Rnd3 protein in human adenomyosis and its potential clinical effect.Methods The immunohistochemical method was employed to detect RhoE/Rnd3 protein expression of the eutopic endometrium obtained from 32 patients without adenomyosis(the control group),and the eutopic(A group) and ectopic(B group)endometrial tissues surgically obtained from 38 patients with adenomyosis(the experimental group).Expression was detected in endometrial glandular cells,stromal cells and smooth muscle cells of the normal and the adenomyosis groups.Results ① In endometrial glandular cells: expressions of RhoE/Rnd3 in the control group were significantly higher than those of the eutopic(t=11.3,P<0.05) and the ectopic(t=9.33,P<0.05) endometria of the experimental groups,but there was no significant difference between the latter two groups(t=1.63,P>0.05).②In stromal and smooth muscle cells there was no significant variance of RhoE /Rnd3 in the stromal cells of the three groups,and there was no significant difference in the smooth muscle cells between the control group and the experimental group(P>0.05).③ Expression of RhoE/Rnd3 in endometrial glandular cells was related to the course of adenomyosis.Expression levels in tissues from patients whose clinical courses exceeded two years were obviously lower than those from patients whose clinical courses were less than two years(P<0.05).Conclusion The RhoE/Rnd3 protein may play a considerable role in the development of adenomyosis,and it could be an important tumor-suppressing gene.