原花青素对瘦素诱导肝星状细胞增殖和TIMP-1产生的影响

目的 观察原花青素(PC)对瘦素诱导的肝星状细胞(HSC)增殖及组织抑制基质金属蛋白酶 1(TIMP 1)产生的影响。方法 磺基罗丹明B法检测HSC增殖，利用活性氧(reactive oxygen species，ROS)探针2,7 二氯二氢荧光素二乙酯检测ROS，酶联免疫吸附实验测定TIMP 1的浓度。结果 PC在50、100、200μg/mL时能够抑制瘦素诱导的HSC的增殖(P<0.05)，同时能够抑制瘦素诱导的ROS的产生(P<0.05)，上述PC各浓度组亦能够抑制TIMP 1的产生(P<0.05)，浓度之间抑制效果具有统计学差异(P<0.05)。结论 PC可能通过抑制瘦素诱导的氧化应激，抑制HSC的增殖以及TIMP 1的产生。

Effects of procyanidins on the proliferation of hepatic stellate cells and production of TIMP-1 induced by leptin

Objective To observe the impacts of procyanidins(PC) on the proliferation of hepatic stellate cell(HSC) and production of tissue inhibitor of matrix metalloprotease-1(TIMP-1) induced by leptin.Methods Sulforhodamine B assay was performed to test the proliferation of HSC.The probe of reactive oxygen species(ROS) 2,7-dichlorfluorescein-diacetate was used to detect the production of ROS.Enzyme-linked immunosorbent assay was used to detect the concentration of TIMP-1.Results PC at concentrations of 50,100,200 μg/mL could inhibit the proliferation of HSC induced by leptin(P<0.05).Meanwhile,it had the inhibitory effect on the production of ROS(P<0.05).The PC groups above could also inhibit the generation of TIMP-1(P<0.05).There were statistically significant differences among the groups(P<0.05).Conclusion PC might inhibit the HSC proliferation and TIMP-1 production through inhibiting the oxidative stress induced by leptin.