排铅聚糖对染铅小鼠的驱铅作用及对必需元素的影响

目的 观察排铅聚糖对醋酸铅染毒小鼠的驱铅作用及对体内必需元素的影响。方法 以昆明种小鼠为实验对象，按体质量随机分为阴性对照组，醋酸铅模型组，排铅聚糖高(1600mg/kg)、中(800mg/kg)、低(400mg/kg)剂量组，除阴性对照组外均腹腔注射醋酸铅溶液染毒造成中毒模型，后各剂量组用不同剂量排铅聚糖灌胃，阴性对照组及醋酸铅模型组用生理盐水灌胃。实验结束后取小鼠的血液、肝、肾、骨、脑组织进行消解，采用原子吸收分光光度法检测血液及各组织中铅及钙、镁、铁、锌、铜、锰的含量。结果 血液及各组织脏器中铅的含量，排铅聚糖各剂量组均低于醋酸铅模型组(P<0.05)。排铅聚糖高剂量组血液、骨中镁的含量低于阴性对照组(P<0.05)，肝铁含量中、高剂量组高于醋酸铅模型组(P<0.05)，而与阴性对照组比较差异无统计学意义(P>0.05)，排铅聚糖各剂量组中钙、铜、锌、锰与阴性对照组相比，在肝、肾、骨、脑中含量差异大部分无统计学意义(P>0.05)。结论 排铅聚糖能有效地排出血液、肝、肾、骨、脑中的铅，但没有明显的剂量 效应关系。用排铅聚糖排出小鼠体内的铅时，除高剂量对血镁、骨镁有促排作用外，对机体内其他必需元素基本无损失，同时中、高剂量对肝内的铁有保护作用。

Effect of lead-excretion glycan on removing lead and essential metal elements in mice

Objective To study the effect of lead-excretion glycan on lead-poisoned mice.Methods 50 healthy mice of Kunming strain(25 males and 25 females) were randomly divided into 5 groups according to their body weights: the negative control group,lead acetate,the lead acetate,model group,the high-dose group(1 600 mg/kg),the medium-dose group(800 mg/kg),and the low-dose group(400 mg/kg).Pb(Ac)2 solutions were given to the groups except the negative control group to form lead-poisoned models in mice,then the low-dose group,the medium-dose group and the high-dose group were given various dose of lead-excretion glycan.The negative control group and the model group were given the normal saline.The mice were sacrificed by decapitation after the last administration.The contents of lead essential elements in blood,liver,kidney,bone and brain were determined by graphite furnace atomic absorption spectrophotometer.Results The contents of lead in blood,liver,kidney,bone and brain in the treated groups were all lower than that in the model group(P<0.05).Magnesiumcontents of the high-dose group in blood and bone were lower than that of the negative control group(P<0.05).Iron contents of the medium and high dose groups in liver was higher than that of model group(P<0.05).There was no statistically significant difference between the negative group and various dose groups in the content of calcium,copper zinc and manganese in liver,kidney,bone and brain(P>0.05).Conclusion Lead-excretion glycan can effectively eliminate lead from lead-poisoned mice,but there is no significant dose-effect relationship.Lead-excretion glycan has no effect on other essential metal elements except that the high dose glycan can eliminate magnesium from blood and bone.The medium and high dose glycan can protect iron in the liver.