腺苷受体A3在特发性炎症性肌病中的表达

目的探讨腺苷受体A3(A3AR)在特发性炎症性肌病(IIMs)发病机制中的作用。方法收集IIMs患者骨骼肌活检组织标本30例,其中皮肌炎(DM)患者15例、多发性肌炎(PM)患者11例、包涵体肌炎(IBM)患者4例;正常对照标本12例。在冰冻切片上行组织化学染色和A3AR抗体免疫荧光染色。结果 A3AR抗体免疫荧光染色阳性见于骨骼肌2型肌纤维胞浆,1型肌纤维胞浆呈阴性;与正常对照比较,30例IIMs患者中22例2型肌纤维胞浆荧光增强。肌纤维膜荧光染色在正常对照呈阴性,而19例IIMs患者呈阳性。血管平滑肌和部分坏死肌纤维A3AR抗体免疫荧光染色呈强阳性。结论人骨骼肌2型肌纤维表达A3AR;特发性炎症性肌病患者A3AR表达增加,可能对肌纤维的修复和凋亡起双向调节作用。

A3 adenosine receptor expression in idopathic inflammatory myopathies

Objective To investigate the effects of A3 adenosine receptor(A3AR)expression in the pathogenesis of idopathic inflammatory myopathies(IIMs).Methods Muscle tissues from 30 cases of IIMs including 15 cases of dermatomyositis(DM),11 cases of polymyositis(PM),4 cases of inclusion body myositis(IBM) and 12 cases of normal controls were collected.Histochemical staining and immunofluorescent staining of the A3AR antibody were used to detect the existence of A3AR in the muscle biopsies.Results The cytosolic immunofluorescent staining of A3AR antibody was positive in the type Ⅱ muscle fibers,however,the staining was absent in the typeⅠmuscle fibers.22 cases among the 30 cases of IIMs displayed a more intense cytosolic staining compared with that of the controls.Cell membrane fluorescent staining was positive in 19 cases of the IIMs,but it was negative in the controls.The highest intensity of the immunofluorescent staining for the A3AR antibody was localized in vascular smooth muscles and some necrotic fibers.Conclusions The A3AR expresses in human type Ⅱ skeletal muscle fibers.Expression of A3AR increases in idopathic inflammatory myopathies(IIMs) patients,and it may have a bidirectional regulation in the repairing and apoptosis of muscle fibers.