FTO rs9939609多态性与2型糖尿病合并冠心病的相关性

目的探讨脂肪量和肥胖相关因子基因(FTO)rs9939609单核苷酸多态性(SNP)与2型糖尿病合并冠心病的相关性。方法随机选择2型糖尿病患者518例,其中单纯2型糖尿病者256例(T2DM组),2型糖尿病合并冠心病者262例(T2DM+CHD组),正常对照组(NC组)242例。所有研究对象应用TaqMan-MGB探针等位基因特异性杂交分析法检测FTO rs9939609单核苷酸多态性,计算等位基因分布频率,比较各组间临床指标差异。结果 FTO rs9939609 TT、TA、AA 3种基因型在该人群的表达频率分别为75.92%、22.37%、1.71%,A等位基因频率为12.89%。T2DM组及T2DM+CHD组FTO基因rs9939609 A等位基因频率显著高于NC组(P<0.05)。A等位基因携带者患2型糖尿病合并冠心病的风险为T等位基因携带者的1.62倍(OR=1.62,95%CI:1.04～2.35,P<0.05)。结论 FTO基因rs9939609多态性与2型糖尿病合并冠心病有相关性,该位点A等位基因可能是2型糖尿病合并冠心病的易感基因之一。

Correlation between FTO rs9939609 polymorphism and type 2 diabetes mellitus patients with coronary heart disease

Objective To study the correlation between the single nucleotide polymorphism of rs9939609 in FTO(fat mass and obesity-related gene) and type 2 diabetes mellitus(T2DM) patients with coronary heart disease(CHD).Methods Genotyping of FTO rs9939609 polymorphism was performed in 518 T2DM patients including 256 simple T2DM patients,262 T2DM with CHD patients and 242 normal controls from the Hospital Affiliated to Jining Medical College using an allelic discrimination assay with the TaqMan-MGB probes.Gene frequencies were calculated and the clinical parameters of the three groups were compared.Results Genotype frequencies of TT,TA and AA of FTO rs9939609 in these people were 75.92%,22.37% and 1.71%,respectively.The frequency for carrying the A allele was 12.89%.The frequency of the A allele was significantly higher in both T2DM and T2DM+CHD groups than that in the control group(P<0.05).The carriers of the A allele had significantly more risk of T2DM+CHD than the carriers of the T allele(OR=1.62,95%CI: 1.04-2.35,P<0.05).Conclusion The single nucleotide polymorphism of FTO gene rs9939609 is associated with T2DM+CHD.These results indicate that the A allele polymorphism of rs9939609 site in FTO gene may be one of the risk genes for T2DM+CHD.