PPARα和IL-1βmRNA在妊娠期子宫肌层的表达及相关性

目的 探讨人类妊娠的不同阶段过氧化物酶体增殖物激活受体α(PPARα)和白细胞介素-1β( IL-1β)mRNA在子宫肌层的表达,以及在维持妊娠、发动分娩中的作用.方法 收集10例未孕(NP组)、10例未足月未临产(PL组)、20例足月未临产(TNL组)及20例足月临产(TL组)育龄期妇女的子宫平滑肌组织,采用荧光定量(real time) PCR技术检测PPARα及IL-1βmRNA的表达.以NP组作为对照,采用2△△CT法分析目的基因组间差异性表达.结果 PPARα在PL组的表达较其他各组均明显升高(P＜0.05),在TL组的表达降低(P＜0.05).IL-1β.在PL组的表达明显降低(P＜0.05),在TL组的表达与其他各组比较则明显升高(P＜0.05).TNL组PPARα及IL-1β的表达与NP组比较差异均无统计学意义(P＞0.05).PPARα与IL-1β呈负相关(r=-0.767,P＜0.05).结论 PPARα及IL-1β在妊娠的不同时期均有明显变化.抗炎因子PPARα可能在维持妊娠中起重要作用,促炎因子IL-1ββ可能参与分娩发动.

Expressions of peroxisome proliferator activated receptor alpha and interleukin-1 beta mRNA in human pregnant myometrium

Objective To explore the expressions of peroxisome proliferator activated receptor alpha(PPARα)and interleukin-1 beta(IL-1β)mRNA in the human pregnant myometrium,and to investigate their roles in maintaining pregnancy or initiating labor.Methods Myometrium was obtained from women in the following four groups: non-pregnant(NP)(n=10),preterm not in labor(PL)(n=10,gestation range 20-35 weeks),term not in labor(TNL)(n=20,gestation range 37-41 weeks) and term in labor(TL)(n=20,gestation range 37-41weeks).PPARα and IL-1β mRNA expressions were quantified by the real-time fluorescent quantitative PCR in the women myometrium.Non-pregnant myometrium was chosen as the control,and the changes and relationship of PPARα and IL-1β in different groups were analyzed by the 2-ΔΔCT relative quantification method.Results Compared to other groups,the expression level of PPARα in group PL was significantly higher(P<0.05),however the expression level in group TL was remarkably lower(P<0.05).The expression level of IL-1β in group PL was much lower than the other three groups(P<0.05),but the expression level in TL group obviously increased(P<0.05).There were no significant differences of the PPARα and IL-1β expressions between groups NP and TNL(P>0.05).PPARα expression was negatively correlated to the IL-1β expression(r=-0.767,P<0.05).Conclusion The expressions of PPARα and IL-1β obviously change in the human pregnant myometrium.Activation of PPARα may have a role to maintain pregnancy,and IL-1β may play a role in initiating labor.